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    nfuA iron-sulfur cluster carrier protein NfuA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947925, updated on 3-Dec-2024

    Summary

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    Official Symbol
    nfuA
    Official Full Name
    iron-sulfur cluster carrier protein NfuA
    Primary source
    ECOCYC:G7748
    Locus tag
    b3414
    See related
    ASAP:ABE-0011144
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3401; gntY; yhgI
    Summary
    NfuA can transfer its [4Fe-4S] cluster into apo-aconitase AcnA and nfuA mutants have reduced survival to oxidative and iron stress (Angelini, 2008). nfuA(yhgI) is required for the utilization of DNA as a carbon source and is a H. influenzae competence protein ComG homolog (Palchevskiy, 2006). nfuA(gntY) does not have a role in gluconate metabolism (T.Conway, personal communication, 2000). iscA, sufA, erpA and nfuA are paralogs, but GntY has an additional thioredoxin-like C-terminal domain. [More information is available at EcoGene: EG12935]. NfuA is an iron-sulfur protein which is able to transfer its [4Fe-4S] iron-sulfur cluster to apo-aconitase in vitro, thus identifying it as an iron-sulfur cluster scaffold protein . [More information is available at EcoCyc: G7748].
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    Genomic context

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    Sequence:
    NC_000913.3 (3545624..3546199)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene pimeloyl-acyl carrier protein methyl ester esterase Neighboring gene DNA utilization protein YhgH Neighboring gene high-affinity gluconate transporter Neighboring gene 4-alpha-glucanotransferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Escherichia coli nfuA is essential for maintenance of Shiga toxin phage Min27 lysogeny under iron-depleted condition. Cao D, et al. FEMS Microbiol Lett, 2015 Oct. PMID 26337151
    2. Molecular organization, biochemical function, cellular role and evolution of NfuA, an atypical Fe-S carrier. Py B, et al. Mol Microbiol, 2012 Oct. PMID 22966982
    3. Destruction and reformation of an iron-sulfur cluster during catalysis by lipoyl synthase. McCarthy EL, et al. Science, 2017 Oct 20. PMID 29051382, Free PMC Article
    4. Physical and functional interactions of a monothiol glutaredoxin and an iron sulfur cluster carrier protein with the sulfur-donating radical S-adenosyl-L-methionine enzyme MiaB. Boutigny S, et al. J Biol Chem, 2013 May 17. PMID 23543739, Free PMC Article
    5. DNA as a nutrient: novel role for bacterial competence gene homologs. Finkel SE, et al. J Bacteriol, 2001 Nov. PMID 11591672, Free PMC Article

    See all (17) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. E. coli K12 MG1655 and its lysogenic strain carrying Stx2-encoding PhiMin27 followed by gene deletion, we provided clear evidence here that NfuA plays an important role in Fe metabolism and maintenance of Stx prophagy lysogeny.
      Title: Escherichia coli nfuA is essential for maintenance of Shiga toxin phage Min27 lysogeny under iron-depleted condition.
    2. GrxD and NfuA could play complementary roles in maturation and repair of MiaB
      Title: Physical and functional interactions of a monothiol glutaredoxin and an iron sulfur cluster carrier protein with the sulfur-donating radical S-adenosyl-L-methionine enzyme MiaB.
    3. In vivo, NfuA is necessary for full activity of complex I under aerobic growth conditions, and of AcnB in the presence of superoxide.
      Title: Molecular organization, biochemical function, cellular role and evolution of NfuA, an atypical Fe-S carrier.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 4 iron, 4 sulfur cluster binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 4 iron, 4 sulfur cluster binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables iron ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables iron-sulfur cluster carrier activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within carbon utilization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within cellular response to iron ion starvation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within iron-sulfur cluster assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in iron-sulfur cluster assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein maturation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein maturation by [4Fe-4S] cluster transfer IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within protein maturation by [4Fe-4S] cluster transfer IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within protein maturation by iron-sulfur cluster transfer IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within response to oxidative stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    iron-sulfur cluster carrier protein NfuA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3545624..3546199
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417873.1 iron-sulfur cluster carrier protein NfuA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417873.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376MJW1
      Conserved Domains (1) summary
      PRK11190
      Location:1 → 191
      PRK11190; iron-sulfur cluster biogenesis protein NfuA
    Nucleotide Protein
    Heading Accession and Version

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