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    ilvN acetohydroxy acid synthase I subunit IlvN [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948183, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ilvN
    Official Full Name
    acetohydroxy acid synthase I subunit IlvN
    Primary source
    ECOCYC:EG10502
    Locus tag
    b3670
    See related
    ASAP:ABE-0011997
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3661
    Summary
    Bifunctional acetohydroxybutanoate synthase / acetolactate synthase (IlvB/N) carries out both the first step in valine biosynthesis and the second step in isoleucine biosynthesis. [More information is available at EcoCyc: EG10502].
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    Genomic context

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    Sequence:
    NC_000913.3 (3850802..3851092, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene inner membrane protein sensing glucose-6-phosphate Neighboring gene sensory histidine kinase UhpB Neighboring gene DNA-binding transcriptional activator UhpA Neighboring gene acetohydroxy acid synthase I subunit IlvB Neighboring gene ilvBN operon leader peptide

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Crystallographic Structures of IlvN·Val/Ile Complexes: Conformational Selectivity for Feedback Inhibition of Aceto Hydroxy Acid Synthases. Bansal A, et al. Biochemistry, 2019 Apr 16. PMID 30887800, Free PMC Article
    2. Cloning and expression of the ilvB gene of Escherichia coli K-12. Newman T, et al. Mol Gen Genet, 1982. PMID 6181375
    3. Mutant of Escherichia coli K-12 missing acetolactate synthase activity. Guardiola J, et al. J Bacteriol, 1974 Oct. PMID 4608700, Free PMC Article
    4. Purification and subunit composition of acetohydroxyacid synthase I from Escherichia coli K-12. Eoyang L, et al. J Bacteriol, 1984 Jan. PMID 6360995, Free PMC Article
    5. A new map location for the ilvB locus of Escherichia coli. Newman TC, et al. Genetics, 1980 Sep. PMID 7009323, Free PMC Article

    See all (36) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The structural and biophysical data indicate the molecular basis for differential binding of the ligands to acetohydroxy acid synthase I subunit IlvN (IlvN).
      Title: Crystallographic Structures of IlvN·Val/Ile Complexes: Conformational Selectivity for Feedback Inhibition of Aceto Hydroxy Acid Synthases.
    2. the dephospho-form of enzyme IIA(Ntr) is required for derepression of the ilvBN operon encoding acetohydroxy acid synthase I catalysing the first step common to the biosynthesis of the branched-chain amino acids.
      Title: Requirement of the dephospho-form of enzyme IIANtr for derepression of Escherichia coli K-12 ilvBN expression.
    3. AHAS I demonstrates cooperative feedback inhibition by valine, kinetics fit closely to an exclusive binding model. Formation of acetolactate by AHAS I is readily reversible & acetolactate can act as substrate for alternative AHAS I-catalyzed reactions.
      Title: Acetohydroxyacid synthase isozyme I from Escherichia coli has unique catalytic and regulatory properties.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables acetolactate synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables acetolactate synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables acetolactate synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables acetolactate synthase regulator activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in L-valine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within L-valine biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in L-valine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in amino acid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in branched-chain amino acid biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in branched-chain amino acid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in isoleucine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within isoleucine biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in isoleucine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of acetolactate synthase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of acetolactate synthase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    acetohydroxy acid synthase I subunit IlvN
    NP_418126.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3850802..3851092 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418126.1 acetohydroxy acid synthase I subunit IlvN [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418126.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0AAN4SWK7
      Conserved Domains (1) summary
      PRK08178
      Location:1 → 96
      PRK08178; acetolactate synthase 1 small subunit
    Nucleotide Protein
    Heading Accession and Version

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