U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    hsdR type I restriction enzyme EcoKI endonuclease subunit [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948878, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    hsdR
    Official Full Name
    type I restriction enzyme EcoKI endonuclease subunit
    Primary source
    ECOCYC:EG10459
    Locus tag
    b4350
    See related
    ASAP:ABE-0014264
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4340; hsp; hsr
    Summary
    HsdR acts as a chaperone in hsdS mutant to allow DNA binding. [More information is available at EcoGene: EG10459]. HsdR is the restriction endonuclease component of the EcoKI restriction-modification system . [More information is available at EcoCyc: EG10459].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (4583249..4586761, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene nucleoid-associated protein SymE Neighboring gene ncRNA Neighboring gene type I restriction enzyme EcoKI specificity subunit Neighboring gene type I restriction enzyme EcoKI methylase subunit Neighboring gene Type IV methyl-directed restriction enzyme EcoKMrr Neighboring gene P-loop guanosine triphosphatase YjiA

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. pHluorin-assisted expression, purification, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I. Grinkevich P, et al. Acta Crystallogr F Struct Biol Commun, 2016 Sep. PMID 27599856, Free PMC Article
    2. Functional coupling of duplex translocation to DNA cleavage in a type I restriction enzyme. Csefalvay E, et al. PLoS One, 2015. PMID 26039067, Free PMC Article
    3. ArdB Protective Activity for Unmodified λ Phage Against EcoKI Restriction Decreases in UV-Treated Escherichia coli. Balabanov VP, et al. Curr Microbiol, 2019 Nov. PMID 31407052
    4. Purification, crystallization and preliminary X-ray analysis of the HsdR subunit of the EcoR124I endonuclease from Escherichia coli. Lapkouski M, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2007 Jul 1. PMID 17620716, Free PMC Article
    5. Method for isolating restriction- and modificationless mutants of Escherichia coli K-12. Del Giudice L. J Bacteriol, 1979 Jan. PMID 368038, Free PMC Article

    See all (26) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The observed decrease in ArdB protective activity in UV-treated cells is due to its binding to unmodified chromosomal DNA, which decreases effective concentrations of free ArdB molecules available for lambda phage protection against EcoKI restriction enzymes.
      Title: ArdB Protective Activity for Unmodified λ Phage Against EcoKI Restriction Decreases in UV-Treated Escherichia coli.
    2. pHluorin-assisted expression, purification, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I has been reported.
      Title: pHluorin-assisted expression, purification, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I.
    3. The X-ray crystal structure of the complete HsdR subunit from E. coli suggested that the DNA cleavage-triggering mechanism involves interdomain contacts mediated by ATP.
      Title: Functional coupling of duplex translocation to DNA cleavage in a type I restriction enzyme.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables type I site-specific deoxyribonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA restriction-modification system IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA restriction-modification system IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA restriction-modification system IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of type I site-specific deoxyribonuclease complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    type I restriction enzyme EcoKI endonuclease subunit
    NP_418770.2

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4583249..4586761 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418770.2 type I restriction enzyme EcoKI endonuclease subunit [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418770.2

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6D0DG16
      Conserved Domains (1) summary
      PRK11448
      Location:2 → 1170
      hsdR; type I restriction enzyme EcoKI subunit R; Provisional
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.