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    mepA peptidoglycan DD-endopeptidase/peptidoglycan LD-endopeptidase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946812, updated on 3-Dec-2024

    Summary

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    Official Symbol
    mepA
    Official Full Name
    peptidoglycan DD-endopeptidase/peptidoglycan LD-endopeptidase
    Primary source
    ECOCYC:EG10580
    Locus tag
    b2328
    See related
    ASAP:ABE-0007691
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2322
    Summary
    MepA is a penicillin-insensitive D-alanyl-D-alanine (DD) endopeptidase responsible for hydrolyzing D-alanyl-2,6-diaminopimelyl (DAP) as well as DAP-DAP (LD) amide bonds within cell wall peptidoglycan peptide crosslinks. [More information is available at EcoCyc: EG10580].
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    Genomic context

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    Sequence:
    NC_000913.3 (2445560..2446384, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene EF-P-Lys34 hydroxylase Neighboring gene putative transporter YfcA Neighboring gene chorismate synthase Neighboring gene ribosomal protein L3 N(5)-glutamine methyltransferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Mutational analysis of peptidoglycan amidase MepA. Firczuk M, et al. Biochemistry, 2007 Jan 9. PMID 17198381
    2. Purification of penicillin-insensitive DD-endopeptidase, a new cell wall peptidoglycan-hydrolyzing enzyme in Escherichia coli, and its inhibition by deoxyribonucleic acids. Tomioka S, et al. Biochem Biophys Res Commun, 1978 Oct 30. PMID 365181
    3. Escherichia coli murein-DD-endopeptidase insensitive to beta-lactam antibiotics. Keck W, et al. J Bacteriol, 1979 Sep. PMID 383691, Free PMC Article
    4. Evidence for multisite growth of Escherichia coli murein involving concomitant endopeptidase and transpeptidase activities. Burman LG, et al. J Bacteriol, 1983 Oct. PMID 6352683, Free PMC Article
    5. The architecture of the murein (peptidoglycan) in gram-negative bacteria: vertical scaffold or horizontal layer(s)? Vollmer W, et al. J Bacteriol, 2004 Sep. PMID 15342566, Free PMC Article

    See all (31) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. N-terminus verified by Edman degradation on mature peptide
      Title: Cloning and characterization of mepA, the structural gene of the penicillin-insensitive murein endopeptidase from Escherichia coli.
    2. MepA-type peptidases are LAS enzymes
      Title: Peptidoglycan amidase MepA is a LAS metallopeptidase.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables endopeptidase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metalloendopeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metallopeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables peptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables peptidase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables serine-type endopeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within peptidoglycan biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within peptidoglycan metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in peptidoglycan metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within peptidoglycan metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to xenobiotic stimulus IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in outer membrane-bounded periplasmic space IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in periplasmic space IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    peptidoglycan DD-endopeptidase/peptidoglycan LD-endopeptidase

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2445560..2446384 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416831.1 peptidoglycan DD-endopeptidase/peptidoglycan LD-endopeptidase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416831.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T5ZWZ1
      Conserved Domains (1) summary
      COG3770
      Location:1 → 274
      MepA; Murein endopeptidase [Cell wall/membrane/envelope biogenesis]
    Nucleotide Protein
    Heading Accession and Version

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