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    pcm protein-L-isoaspartate O-methyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944923, updated on 3-Dec-2024

    Summary

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    Official Symbol
    pcm
    Official Full Name
    protein-L-isoaspartate O-methyltransferase
    Primary source
    ECOCYC:EG10689
    Locus tag
    b2743
    See related
    ASAP:ABE-0009006
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2738
    Summary
    Pcm may work with SurE: they are co-transcribed and have addictive effects on isoaspartyl accumulation during stationary phase. [More information is available at EcoGene: EG10689]. The pcm-encoded L-isoaspartate protein carboxylmethyltransferase serves to repair abnormal L-isoaspartyl residues that arise sponaneously from aspartyl and asparaginyl residues in proteins. [More information is available at EcoCyc: EG10689].
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    Genomic context

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    Sequence:
    NC_000913.3 (2868893..2869519, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene RNA polymerase sigma factor RpoS Neighboring gene murein hydrolase activator NlpD Neighboring gene broad specificity 5'(3')-nucleotidase and polyphosphatase Neighboring gene tRNA pseudouridine(13) synthase Neighboring gene 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase Neighboring gene 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A protein methyltransferase specific for altered aspartyl residues is important in Escherichia coli stationary-phase survival and heat-shock resistance. Li C, et al. Proc Natl Acad Sci U S A, 1992 Oct 15. PMID 1409717, Free PMC Article
    2. Functional solubilization of aggregation-prone TRAIL protein facilitated by coexpressing with protein isoaspartate methyltranferase. Zhu H, et al. Appl Microbiol Biotechnol, 2006 Oct. PMID 16575568
    3. Purification, gene cloning, and sequence analysis of an L-isoaspartyl protein carboxyl methyltransferase from Escherichia coli. Fu JC, et al. J Biol Chem, 1991 Aug 5. PMID 1860862
    4. Recovery from long-term stationary phase and stress survival in Escherichia coli require the L-isoaspartyl protein carboxyl methyltransferase at alkaline pH. Hicks WM, et al. Microbiology (Reading), 2005 Jul. PMID 16000706
    5. Protein isoaspartate methyltransferase is a multicopy suppressor of protein aggregation in Escherichia coli. Kern R, et al. J Bacteriol, 2005 Feb. PMID 15687202, Free PMC Article

    See all (19) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. N-terminus verified by Edman degradation on mature peptide
      Title: Purification, gene cloning, and sequence analysis of an L-isoaspartyl protein carboxyl methyltransferase from Escherichia coli.
    2. discovered that protein isoaspartate methyltransferase (PIMT) is a multicopy suppressor of preS2-S'-beta-galactosidase aggregation at 43 degrees C.
      Title: Protein isoaspartate methyltransferase is a multicopy suppressor of protein aggregation in Escherichia coli.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein modification process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within protein repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    protein-L-isoaspartate O-methyltransferase
    NP_417223.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2868893..2869519 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417223.1 protein-L-isoaspartate O-methyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417223.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      Q4JH73
      Conserved Domains (1) summary
      PRK00312
      Location:1 → 208
      pcm; protein-L-isoaspartate(D-aspartate) O-methyltransferase
    Nucleotide Protein
    Heading Accession and Version

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