U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    cas1 multifunctional nuclease Cas1 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947228, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    cas1
    Official Full Name
    multifunctional nuclease Cas1
    Primary source
    ECOCYC:G7425
    Locus tag
    b2755
    See related
    ASAP:ABE-0009038
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2750; ygbT
    Summary
    DUF48 member. [More information is available at EcoGene: EG13114]. Cas1: [More information is available at EcoCyc: G7425].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (2878855..2879772, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene alkaline phosphatase isozyme conversion protein Neighboring gene CRISPR-associated endoribonuclease Cas2 Neighboring gene pre-CRISPR RNA endonuclease Neighboring gene type I-E CRISPR system Cascade subunit CasD Neighboring gene type I-E CRISPR system Cascade subunit CasC

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Invasive DNA, chopped and in the CRISPR. Marraffini LA, et al. Structure, 2009 Jun 10. PMID 19523896, Free PMC Article
    2. Spacer-length DNA intermediates are associated with Cas1 in cells undergoing primed CRISPR adaptation. Musharova O, et al. Nucleic Acids Res, 2017 Apr 7. PMID 28204574, Free PMC Article
    3. A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System. Wright AV, et al. Mol Cell, 2019 Feb 21. PMID 30709710, Free PMC Article
    4. Parasite Exposure Drives Selective Evolution of Constitutive versus Inducible Defense. Westra ER, et al. Curr Biol, 2015 Apr 20. PMID 25772450
    5. Structures of the CRISPR genome integration complex. Wright AV, et al. Science, 2017 Sep 15. PMID 28729350, Free PMC Article

    See all (20) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A mini-integrase comprising the type V-C Cas1 protein alone catalyzes DNA integration with a preference for short (17- to 19-base-pair) DNA fragments in a two-protein-type V-C CRISPR system.
      Title: A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.
    2. These results explain how the Cas1-Cas2 CRISPR integrase recognizes a sequence-dependent DNA structure to ensure site-selective CRISPR array expansion during the initial step of bacterial adaptive immunity.
      Title: Structures of the CRISPR genome integration complex.
    3. in Escherichia coli cells undergoing primed adaptation, spacer-sized fragments of foreign DNA are associated with Cas1.
      Title: Spacer-length DNA intermediates are associated with Cas1 in cells undergoing primed CRISPR adaptation.
    4. to form a long-lived ternary complex containing Cas1-Cas2, the acceptor DNA must carry a CRISPR locus, and the protospacer must not contain 3-single-stranded overhangs longer than 5 bases.
      Title: DNA binding specificities of Escherichia coli Cas1-Cas2 integrase drive its recruitment at the CRISPR locus.
    5. Asymmetric positioning of Cas1-Cas2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system.
      Title: Asymmetric positioning of Cas1-2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system.
    6. Cas1 from both Escherichia coli and Sulfolobus solfataricus display sequence specific activity, with a clear preference for the nucleotides flanking the integration site at the leader-repeat 1 boundary of the CRISPR locus.
      Title: Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition.
    7. These results establish Cas1-Cas2 as a trans-acting factor necessary for the recruitment and regulation of Cas3 at escape targets. Based on findings, a mechanistic framework is proposed describing how Cascade, Cas1, Cas2, and Cas3 work together to process and disable foreign genetic elements.
      Title: Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System.
    8. To understand the molecular mechanisms of spacer acquisition, the crystal structure of E. coli Cas1-Cas2 bound with dual-forked DNA was determined; structure highlights the mechanistic principles related to new spacer acquisition, the protospacer DNA captured by Cas1-Cas2 adopts a dual-forked form, with the 3' overhangs of the protospacer essential for new spacer.
      Title: Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems.
    9. CRISPR-Cas functions by integrating phage sequences into CRISPR loci on the host genome.
      Title: Parasite Exposure Drives Selective Evolution of Constitutive versus Inducible Defense.
    10. Cas1-Cas2 complexes specify sites of CRISPR spacer integration.
      Title: Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity.
    Submit: New GeneRIF Correction See all GeneRIFs (11)

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    General protein information

    Go to the top of the page Help
    Preferred Names
    multifunctional nuclease Cas1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2878855..2879772 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417235.1 multifunctional nuclease Cas1 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417235.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T5YJ48
      Conserved Domains (1) summary
      TIGR03638
      Location:6 → 272
      cas1_ECOLI; CRISPR-associated endonuclease Cas1, subtype I-E/ECOLI
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.