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    lysU lysine--tRNA ligase/Ap4A synthetase/Ap3A synthetase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948645, updated on 17-Dec-2024

    Summary

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    Official Symbol
    lysU
    Official Full Name
    lysine--tRNA ligase/Ap4A synthetase/Ap3A synthetase
    Primary source
    ECOCYC:EG10553
    Locus tag
    b4129
    See related
    ASAP:ABE-0013520
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4123
    Summary
    The lysyl-tRNA synthetase LysU is a member of the family of aminoacyl tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. [More information is available at EcoCyc: EG10553].
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    Genomic context

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    Sequence:
    NC_000913.3 (4353200..4354717, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene antitoxin of the GhoTS toxin-antitoxin system Neighboring gene toxin of the GhoTS toxin-antitoxin system Neighboring gene dipeptide/tripeptide:H(+) symporter DtpC Neighboring gene lysine decarboxylase 1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Functional asymmetry in the lysyl-tRNA synthetase explored by molecular dynamics, free energy calculations and experiment. Hughes SJ, et al. BMC Struct Biol, 2003 Jun 4. PMID 12787471, Free PMC Article
    2. The crystal structure of the lysyl-tRNA synthetase (LysU) from Escherichia coli. Onesti S, et al. Structure, 1995 Feb 15. PMID 7735833
    3. Regulation of lrp gene expression by H-NS and Lrp proteins in Escherichia coli: dominant negative mutations in lrp. Oshima T, et al. Mol Gen Genet, 1995 Jun 10. PMID 7603430
    4. Multiple forms of lysyl-transfer ribonucleic acid synthetase in Escherichia coli. Hirshfield IN, et al. J Bacteriol, 1981 Apr. PMID 7012120, Free PMC Article
    5. Regulation of lysyl-tRNA synthetase expression by histone-like protein H-NS of Escherichia coli. Ito K, et al. J Bacteriol, 1994 Dec. PMID 7961513, Free PMC Article

    See all (47) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. N-terminus verified by Edman degradation on mature peptide
      Title: Homology of lysS and lysU, the two Escherichia coli genes encoding distinct lysyl-tRNA synthetase species.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables aminoacyl-tRNA ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables catalytic activity, acting on a nucleic acid IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lysine-tRNA ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables lysine-tRNA ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lysine-tRNA ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lysine-tRNA ligase activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA capping IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in cellular response to heat IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in lysyl-tRNA aminoacylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in lysyl-tRNA aminoacylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in lysyl-tRNA aminoacylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within lysyl-tRNA aminoacylation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within tRNA aminoacylation for protein translation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol HDA PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane HDA PubMed 

    General protein information

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    Preferred Names
    lysine--tRNA ligase/Ap4A synthetase/Ap3A synthetase
    NP_418553.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4353200..4354717 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418553.1 lysine--tRNA ligase/Ap4A synthetase/Ap3A synthetase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418553.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A3P5E0C6
      Conserved Domains (1) summary
      PRK12445
      Location:1 → 505
      PRK12445; lysyl-tRNA synthetase; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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