putA fused DNA-binding transcriptional repressor/proline dehydrogenase/1-pyrroline-5-carboxylate dehydrogenase PutA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: putA
Official Full Name: fused DNA-binding transcriptional repressor/proline dehydrogenase/1-pyrroline-5-carboxylate dehydrogenase PutA
Primary source: ECOCYC:EG10801
Locus tag: b1014
See related: ASAP:ABE-0003424
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK1005; poaA; putC
Summary: PutA is a multifunctional membrane-associated flavoprotein involved in proline catabolism: the central proline dehydrogenese domain feeds its product to the C-terminal Delta(1)-pyrroline-5-carboxylate dehydrogenese domain, to produce glutamate. [More information is available at EcoGene: EG10801]. The PutA protein is a bifunctional enzyme and a transcriptional repressor of the put (proline utilization) regulon and belongs to the PutA family. [More information is available at EcoCyc: EG10801].
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Genomic context

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Sequence:: NC_000913.3 (1074920..1078882, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Deletion of the C-terminal motif (CCM) or replacement of hydrophobic residues with negatively charged residues within the CCM impairs EcPutA functional and physical membrane association
Title: Discovery of the Membrane Binding Domain in Trifunctional Proline Utilization A.
crystal structures of the proline dehydrogenase domain mutants PutA86-630D370N and PutA86-630D370A complexed with the proline analogue show that the mutations cause only minor perturbations to the active site but no major structural changes
Title: Involvement of the β3-α3 loop of the proline dehydrogenase domain in allosteric regulation of membrane association of proline utilization A.
Data show that PutA is a symmetric V-shaped dimer having dimensions of 205 x 85 x 55 A. The particle consists of two large lobes connected by a 30-A diameter cylinder
Title: Small-angle X-ray scattering studies of the oligomeric state and quaternary structure of the trifunctional proline utilization A (PutA) flavoprotein from Escherichia coli.
kinetic data, along with analysis of crystal structure data for the PRODH domain, suggest that the proline:ubiquinone oxidoreductase reaction occurs via a rapid equilibrium ping-pong mechanism with proline and ubiquinone binding at two distinct sites
Title: Steady-state kinetic mechanism of the proline:ubiquinone oxidoreductase activity of proline utilization A (PutA) from Escherichia coli.
analysis of metabolism and gene expression in the proline utilization A protein from Escherichia coli [review]
Title: Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli.
From this study it is clear that reduction of flavin adenine dinucleotide bound to the proline dehydrogenase active site of PutA elicits global conforamtional changes that direct PutA-membrane associations.
Title: Regulation of PutA-membrane associations by flavin adenine dinucleotide reduction.
identify molecular interactions in the PutA active site that underlie redox-dependent functional switching of PutA
Title: Redox-induced changes in flavin structure and roles of flavin N(5) and the ribityl 2'-OH group in regulating PutA--membrane binding.
The proline dehydrogenase (PRODH)/L-tetrahydro-2-furoic acid complex is the first structure of PRODH with a five-membered ring proline analogue bound in the active site.
Title: Structures of the Escherichia coli PutA proline dehydrogenase domain in complex with competitive inhibitors.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables 1-pyrroline-5-carboxylate dehydrogenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 1-pyrroline-5-carboxylate dehydrogenase activity	IDA Inferred from Direct Assay more info	PubMed
enables 1-pyrroline-5-carboxylate dehydrogenase activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription repressor activity	IDA Inferred from Direct Assay more info	PubMed
enables cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables flavin adenine dinucleotide binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables proline dehydrogenase activity	IDA Inferred from Direct Assay more info	PubMed
enables proline dehydrogenase activity	IEA Inferred from Electronic Annotation more info
enables proline dehydrogenase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in proline biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in proline catabolic process	IEA Inferred from Electronic Annotation more info
involved_in proline catabolic process to glutamate	IBA Inferred from Biological aspect of Ancestor more info
involved_in proline catabolic process to glutamate	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within proline catabolic process to glutamate	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proline metabolic process	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasmic side of plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: fused DNA-binding transcriptional repressor/proline dehydrogenase/1-pyrroline-5-carboxylate dehydrogenase PutA

NP_415534.1

EC 1.2.1.88

EC 1.5.5.2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

1074920..1078882 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_415534.1 fused DNA-binding transcriptional repressor/proline dehydrogenase/1-pyrroline-5-carboxylate dehydrogenase PutA [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_415534.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A6N8QZP8, A0A8T5ZYY9

Conserved Domains (1) summary

PRK11809
Location:1 → 1320

putA; trifunctional transcriptional regulator/proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase; Reviewed