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    rbfA 30S ribosome binding factor [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947685, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rbfA
    Official Full Name
    30S ribosome binding factor
    Primary source
    ECOCYC:EG11178
    Locus tag
    b3167
    See related
    ASAP:ABE-0010406
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3156; sdr-43; yhbB
    Summary
    RbfA can be found associated with the 30S subunit of the ribosome and is essential for efficient processing of the 16S rRNA . [More information is available at EcoCyc: EG11178].
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    Genomic context

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    Sequence:
    NC_000913.3 (3312777..3313178, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene 30S ribosomal subunit protein S15 Neighboring gene tRNA pseudouridine(55) synthase Neighboring gene translation initiation factor IF-2beta' Neighboring gene transcription termination/antitermination protein NusA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Escherichia coli rimM and yjeQ null strains accumulate immature 30S subunits of similar structure and protein complement. Leong V, et al. RNA, 2013 Jun. PMID 23611982, Free PMC Article
    2. Solution NMR structure of ribosome-binding factor A (RbfA), a cold-shock adaptation protein from Escherichia coli. Huang YJ, et al. J Mol Biol, 2003 Mar 21. PMID 12628255
    3. RbfA and IF3 couple ribosome biogenesis and translation initiation to increase stress tolerance. Sharma IM, et al. Nucleic Acids Res, 2020 Jan 10. PMID 31728529, Free PMC Article
    4. RbfA, a 30S ribosomal binding factor, is a cold-shock protein whose absence triggers the cold-shock response. Jones PG, et al. Mol Microbiol, 1996 Sep. PMID 8898389
    5. The C-terminal helix in the YjeQ zinc-finger domain catalyzes the release of RbfA during 30S ribosome subunit assembly. Jeganathan A, et al. RNA, 2015 Jun. PMID 25904134, Free PMC Article

    See all (25) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. An Alternative Role of RluD in the Fidelity of Translation Initiation in Escherichia coli.
      Title: An Alternative Role of RluD in the Fidelity of Translation Initiation in Escherichia coli.
    2. By gating the transition from 30S ribosome biogenesis to translation initiation, RbfA and IF3 maintain the fidelity of bacterial protein synthesis.
      Title: RbfA and IF3 couple ribosome biogenesis and translation initiation to increase stress tolerance.
    3. Results shows that YjeQ promotes the release of RbfA through the C-terminal helix in its zinc-finger domain.
      Title: The C-terminal helix in the YjeQ zinc-finger domain catalyzes the release of RbfA during 30S ribosome subunit assembly.
    4. Overexpression of RbfA in the absence of the KsgA results in impaired translation initiation.
      Title: Overexpression of RbfA in the absence of the KsgA checkpoint results in impaired translation initiation.
    5. N-terminus verified by Edman degradation on mature peptide
      Title: '98 Escherichia coli SWISS-2DPAGE database update.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ribosomal small subunit binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ribosomal small subunit binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA damage response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in maturation of SSU-rRNA IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within maturation of SSU-rRNA IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within maturation of SSU-rRNA IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to cold IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within ribosomal small subunit biogenesis IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within ribosomal small subunit biogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ribosome biogenesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ribosome biogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IC
    Inferred by Curator
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    30S ribosome binding factor

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3312777..3313178 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417636.1 30S ribosome binding factor [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417636.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A192CCL0, A0AA36L0U8
      Conserved Domains (1) summary
      PRK00521
      Location:1 → 123
      rbfA; 30S ribosome-binding factor RbfA
    Nucleotide Protein
    Heading Accession and Version

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