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    hflX ribosome rescue factor HflX [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948688, updated on 3-Dec-2024

    Summary

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    Official Symbol
    hflX
    Official Full Name
    ribosome rescue factor HflX
    Primary source
    ECOCYC:EG10437
    Locus tag
    b4173
    See related
    ASAP:ABE-0013661
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4169; hflA; hslX
    Summary
    HflX has both GTPase and ATPase activities (Jain, 2009; Dutta, 2009). [More information is available at EcoGene: EG10437]. HflX has a GTP-binding/GTPase region , and it may be involved in regulation of HflC and HflK activity . [More information is available at EcoCyc: EG10437].
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    Genomic context

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    Sequence:
    NC_000913.3 (4400672..4401952)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA mismatch repair protein MutL Neighboring gene tRNA dimethylallyltransferase Neighboring gene RNA-binding protein Hfq Neighboring gene regulator of FtsH protease Neighboring gene regulator of FtsH protease

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The conserved GTPase HflX is a ribosome splitting factor that binds to the E-site of the bacterial ribosome. Coatham ML, et al. Nucleic Acids Res, 2016 Feb 29. PMID 26733579, Free PMC Article
    2. HflX protein protects Escherichia coli from manganese stress. Sengupta S, et al. J Biosci, 2018 Dec. PMID 30541959
    3. Conserved P-loop GTPases of unknown function in bacteria: an emerging and vital ensemble in bacterial physiology. Brown ED. Biochem Cell Biol, 2005 Dec. PMID 16333325
    4. Fine structure mapping, complementation, and physiology of Escherichia coli hfl mutants. Gautsch JW, et al. Genetics, 1974 Jul. PMID 4606553, Free PMC Article
    5. Identification and sequence determination of the host factor gene for bacteriophage Q beta. Kajitani M, et al. Nucleic Acids Res, 1991 Mar 11. PMID 2020545, Free PMC Article

    See all (28) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. cryo-electron microscopy structure of the 50S-HflX complex in the presence of nonhydrolyzable analogues of ATP and guanosine triphosphate hints at a mode of action for the RNA helicase and suggests the linker helical domain may have a determinant role in RNA unwinding. Heat stress results in inactivation of the ribosome, and we show that HflX can restore heat-damaged ribosomes and improve cell survival.
      Title: The universally conserved GTPase HflX is an RNA helicase that restores heat-damaged Escherichia coli ribosomes.
    2. HflX protein protects Escherichia coli from manganese stress.
      Title: HflX protein protects Escherichia coli from manganese stress.
    3. Furthermore, we provide evidence that the bound state of the peptidyl transferase centre is able to regulate the GTPase activity of E-site bound HflX
      Title: The conserved GTPase HflX is a ribosome splitting factor that binds to the E-site of the bacterial ribosome.
    4. These results suggest a primary role of HflX in rescuing translationally arrested ribosomes under stress conditions.
      Title: HflX is a ribosome-splitting factor rescuing stalled ribosomes under stress conditions.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables GTPase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTPase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables guanosine tetraphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables guanosine tetraphosphate binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables rRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ribosomal large subunit binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ribosome binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ribosome binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within rescue of stalled ribosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within response to heat IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to heat IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within ribosome disassembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within ribosome disassembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    NOT acts_upstream_of_or_within viral latency IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ribosome rescue factor HflX
    NP_418594.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4400672..4401952
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418594.1 ribosome rescue factor HflX [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418594.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AM10
      Conserved Domains (1) summary
      PRK11058
      Location:1 → 426
      PRK11058; GTPase HflX; Provisional
    Nucleotide Protein
    Heading Accession and Version

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