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    rsmA 16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944939, updated on 17-Dec-2024

    Summary

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    Official Symbol
    rsmA
    Official Full Name
    16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase
    Primary source
    ECOCYC:EG10523
    Locus tag
    b0051
    See related
    ASAP:ABE-0000175
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0052; ksgA
    Summary
    RsmA(KsgA) dimethylates two adjacent 16S rRNA adenosines. rsmA(ksgA) deletion mutants grow more slowly than an isogenic wt at 20C (Connolly, 2008). [More information is available at EcoGene: EG10523]. KsgA is the methyltransferase responsible for dimethylation of 16S rRNA at the two adjacent adenosine bases A1518 and A1519 . [More information is available at EcoCyc: EG10523].
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    Genomic context

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    Sequence:
    NC_000913.3 (51609..52430, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene diadenosine tetraphosphatase Neighboring gene DUF525 domain-containing protein ApaG Neighboring gene 4-hydroxythreonine-4-phosphate dehydrogenase Neighboring gene chaperone SurA Neighboring gene lipopolysaccharide assembly protein LptD

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structural basis of successive adenosine modifications by the conserved ribosomal methyltransferase KsgA. Stephan NC, et al. Nucleic Acids Res, 2021 Jun 21. PMID 34086932, Free PMC Article
    2. Crystallization and preliminary X-ray diffraction analysis of KsgA, a universally conserved RNA adenine dimethyltransferase in Escherichia coli. O'Farrell HC, et al. Acta Crystallogr D Biol Crystallogr, 2003 Aug. PMID 12876362
    3. Crystal structure of KsgA, a universally conserved rRNA adenine dimethyltransferase in Escherichia coli. O'Farrell HC, et al. J Mol Biol, 2004 May 28. PMID 15136037
    4. Some properties of the ribosomal RNA methyltransferase encoded by ksgA and the polarity of ksgA transcription. Andrésson OS, et al. Mol Gen Genet, 1980. PMID 7005624
    5. The adenosine dimethyltransferase KsgA recognizes a specific conformational state of the 30S ribosomal subunit. Desai PM, et al. Arch Biochem Biophys, 2006 May 15. PMID 16620761

    See all (43) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural basis of successive adenosine modifications by the conserved ribosomal methyltransferase KsgA.
      Title: Structural basis of successive adenosine modifications by the conserved ribosomal methyltransferase KsgA.
    2. Overexpression of RbfA in the absence of the KsgA results in impaired translation initiation.
      Title: Overexpression of RbfA in the absence of the KsgA checkpoint results in impaired translation initiation.
    3. A key aspect of KsgA's mechanism for regulating ribosome biogenesis is the ability of the enzyme to dimethylate its two target adenosines.
      Title: Control of substrate specificity by a single active site residue of the KsgA methyltransferase.
    4. Helix 24 and helix 45 of KsgA play important roles in catalysis of 16S rRNA.
      Title: Site-directed mutants of 16S rRNA reveal important RNA domains for KsgA function and 30S subunit assembly.
    5. KsgA functions as a late-stage ribosome biogenesis factor, and methylation is a trigger for release of KsgA from the assembling subunits. Gene deletion leads to cold sensitivity and altered ribosomal profiles.
      Title: Mechanistic insight into the ribosome biogenesis functions of the ancient protein KsgA.
    6. This study suggests that KsgA has another unidentified function that plays a role in the suppression of the cold-sensitive phenotype of the Era(E200K) strain and that this function may be involved in the acid shock response.
      Title: Dissection of 16S rRNA methyltransferase (KsgA) function in Escherichia coli.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables double-stranded DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables mRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables rRNA (adenine) methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables rRNA (adenine-N6,N6-)-dimethyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables rRNA (adenine-N6,N6-)-dimethyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables rRNA (adenine-N6,N6-)-dimethyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ribosomal small subunit binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within maturation of SSU-rRNA IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in rRNA base methylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rRNA methylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in rRNA modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in rRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within rRNA processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to antibiotic IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within ribosomal small subunit assembly IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within ribosomal small subunit assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase
    NP_414593.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      51609..52430 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414593.1 16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414593.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6C9Q9Q7
      Conserved Domains (1) summary
      PRK00274
      Location:1 → 267
      ksgA; 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA
    Nucleotide Protein
    Heading Accession and Version

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