polB DNA polymerase II [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: polB
Official Full Name: DNA polymerase II
Primary source: ECOCYC:EG10747
Locus tag: b0060
See related: ASAP:ABE-0000202
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: dinA; ECK0061
Summary: DNA polymerase II (Pol II) is a combined polymerase and exonuclease involved in replication restart following UV exposure, translesion synthesis and nucleotide excision repair. [More information is available at EcoCyc: EG10747].
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Genomic context

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Sequence:: NC_000913.3 (63429..65780, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

polB gene expression is controlled by SOS and RpoS regulons.
Title: The SOS and RpoS Regulons Contribute to Bacterial Cell Robustness to Genotoxic Stress by Synergistically Regulating DNA Polymerase Pol II.
Polymerases II, IV and V may bypass trans-4-hydroxy-2-nonenal modified bases by translesion synthesis and restore DNA synthesis beyond the arrested site, although with different efficiencies.
Title: Role of damage-specific DNA polymerases in M13 phage mutagenesis induced by a major lipid peroxidation product trans-4-hydroxy-2-nonenal.
Data report that 15% of stress-induced mutations associated with DNA double-strand break repair in carbon-starved Escherichia coli requires high-fidelity Pol II, and that this component also requires RpoS.
Title: Separate DNA Pol II- and Pol IV-dependent pathways of stress-induced mutation during double-strand-break repair in Escherichia coli are controlled by RpoS.
transcription by single Pol II complexes allows survival of the original H3/H4 histones, while multiple, closely spaced complexes of transcribing Pol II can induce displacement of all core histones along the gene
Title: RNA polymerase complexes cooperate to relieve the nucleosomal barrier and evict histones.
Compared to the replicative B family polymerases, DNA Pol II has subtle amino acid changes remote from the active site that allow it to replicate normal DNA with high efficiency yet conduct translesion synthesis when needed.
Title: Structural insight into translesion synthesis by DNA Pol II.
Data show that an important role is described for DNA Pol II in preventing the mutations and reveal that Pol II is a major editor of Pol III mediated replication errors.
Title: Role of accessory DNA polymerases in DNA replication in Escherichia coli: analysis of the dnaX36 mutator mutant.
elevated expression of Pol-II leads to an approximately 100-fold increase in spontaneous mutagenesis in a manner that is independent of SOS, umuDC, dinB, recA, uvrA and mutS functions
Title: Elevated expression of DNA polymerase II increases spontaneous mutagenesis in Escherichia coli.
N-terminus verified by Edman degradation on mature peptide
Title: DNA polymerase II is encoded by the DNA damage-inducible dinA gene of Escherichia coli.
These data suggest that DNA polymerase II is responsible for the in vivo mutagenic bypass of these lesions in wild-type E. coli.
Title: Evidence for Escherichia coli polymerase II mutagenic bypass of intrastrand DNA crosslinks.
The results fit a model in which Pol II acts as proofreader for HE-produced misinsertion errors.
Title: DNA polymerase II as a fidelity factor in chromosomal DNA synthesis in Escherichia coli.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables 3'-5'-DNA exonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3'-5'-DNA exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables DNA-directed DNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-directed DNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed DNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication proofreading	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within DNA replication proofreading	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within DNA-templated DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in SOS response	IBA Inferred from Biological aspect of Ancestor more info
involved_in SOS response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within SOS response	IEP Inferred from Expression Pattern more info	PubMed

General protein information

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Preferred Names: DNA polymerase II

NP_414602.1

EC 2.7.7.7

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

63429..65780 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_414602.1 DNA polymerase II [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_414602.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A9P2I8B1

Conserved Domains (1) summary

PRK05762
Location:1 → 783

PRK05762; DNA polymerase II; Reviewed