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    frr ribosome-recycling factor [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946122, updated on 17-Dec-2024

    Summary

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    Official Symbol
    frr
    Official Full Name
    ribosome-recycling factor
    Primary source
    ECOCYC:EG10335
    Locus tag
    b0172
    See related
    ASAP:ABE-0000588
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0171; rrf
    Summary
    frr is an essential gene. [More information is available at EcoGene: EG10335]. Rrf recycles the ribosome upon translation termination . [More information is available at EcoCyc: EG10335].
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    Genomic context

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    Sequence:
    NC_000913.3 (192872..193429)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein chain elongation factor EF-Ts Neighboring gene UMP kinase Neighboring gene 1-deoxy-D-xylulose 5-phosphate reductoisomerase Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Emerging understanding of translation termination. Nakamura Y, et al. Cell, 1996 Oct 18. PMID 8861897
    2. Characteristic domain motion in the ribosome recycling factor revealed by 15N NMR relaxation experiments and molecular dynamics simulations. Yoshida T, et al. Biochemistry, 2003 Apr 15. PMID 12680764
    3. Orientation of ribosome recycling factor in the ribosome from directed hydroxyl radical probing. Lancaster L, et al. Cell, 2002 Oct 4. PMID 12372306
    4. Binding of ribosome recycling factor to ribosomes, comparison with tRNA. Hirokawa G, et al. J Biol Chem, 2002 Sep 27. PMID 12138121
    5. Post-termination complex disassembly by ribosome recycling factor, a functional tRNA mimic. Hirokawa G, et al. EMBO J, 2002 May 1. PMID 11980724, Free PMC Article

    See all (77) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Ribosome recycling is not critical for translational coupling in Escherichia coli.
      Title: Ribosome recycling is not critical for translational coupling in Escherichia coli.
    2. The cells with high EFG/RRF (or low RRF/EFG) ratios are more susceptible to fusidic acid than those with low EFG/RRF (or high RRF/EFG) ratios.
      Title: Antimicrobial activity of fusidic acid in Escherichia coli is dependent on the relative levels of ribosome recycling factor and elongation factor G.
    3. Ribosome recycling after peptide chain termination is a special case of the general RRF and EF-G function, which happens whenever fMet-tRNA is not available for triggering 70S scanning.
      Title: The Termination Phase in Protein Synthesis is not Obligatorily Followed by the RRF/EF-G-Dependent Recycling Phase.
    4. The disruption of bridge B2a upon RRF binding to the ribosome seen in the present structures reveals one of the key roles that RRF plays in ribosome recycling, the dissociation of 70S ribosomes into subunits.
      Title: Structural Insights into ribosome recycling factor interactions with the 70S ribosome.
    5. N-terminus verified by Edman degradation on complete protein
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    6. domain II of RRF plays a crucial role in the concerted action of RRF and EF-G for the post-termination complex disassembly, and the specific interaction between RRF and EF-G on ribosomes mainly depends on the interaction between domain II of RRF and EF-G
      Title: Domain II plays a crucial role in the function of ribosome recycling factor.
    7. data enable a model to be proposed for the dual action of EF-G and RRF during ribosome recycling
      Title: X-ray crystallography study on ribosome recycling: the mechanism of binding and action of RRF on the 50S ribosomal subunit.
    8. RRF is capable of spontaneously moving from its initial binding site on the 70S ribosome to a site exclusively on the large 50S ribosomal subunit.
      Title: Progression of the ribosome recycling factor through the ribosome dissociates the two ribosomal subunits.
    9. V117D mutation does not perturb the efficient interdomain interaction, which results in cooperative unfolding of the RRF protein.
      Title: Cooperative unfolding of Escherichia coli ribosome recycling factor originating from its domain-domain interaction and its implication for function.
    10. Data show that monosomes produced in ribosome recycling factor-deficient cells are able to translate synthetic homopolymer but not natural mRNA with leader and canonical initiation signal.
      Title: In vivo effect of inactivation of ribosome recycling factor - fate of ribosomes after unscheduled translation downstream of open reading frame.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ribosomal large subunit binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ribosomal large subunit binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cytoplasmic translational termination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within cytoplasmic translational termination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in translation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translational termination IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ribosome-recycling factor

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      192872..193429
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414714.1 ribosome-recycling factor [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414714.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A0H2YVQ4, A0A2X3KIN3
      Conserved Domains (1) summary
      PRK00083
      Location:1 → 185
      frr; ribosome recycling factor; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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