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    csgA curlin, major subunit [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 949055, updated on 3-Dec-2024

    Summary

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    Official Symbol
    csgA
    Official Full Name
    curlin, major subunit
    Primary source
    ECOCYC:EG11489
    Locus tag
    b1042
    See related
    ASAP:ABE-0003537
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1028
    Summary
    The csgAB operon is regulated by CsgD. [More information is available at EcoGene: EG11489]. The CsgA protein is the major subunit of curlin; it is actively secreted to the extracellular milieu , where CsgA monomers self-assemble into curli . [More information is available at EcoCyc: EG11489].
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    Genomic context

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    Sequence:
    NC_000913.3 (1104447..1104902)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional dual regulator CsgD Neighboring gene curlin, minor subunit Neighboring gene curlin chaperone Neighboring gene uncharacterized protein YmdA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The RpoS sigma factor relieves H-NS-mediated transcriptional repression of csgA, the subunit gene of fibronectin-binding curli in Escherichia coli. Olsén A, et al. Mol Microbiol, 1993 Feb. PMID 8459772
    2. NMR insights into the pre-amyloid ensemble and secretion targeting of the curli subunit CsgA. Sewell L, et al. Sci Rep, 2020 May 12. PMID 32398666, Free PMC Article
    3. Purification and characterization of thin, aggregative fimbriae from Salmonella enteritidis. Collinson SK, et al. J Bacteriol, 1991 Aug. PMID 1677357, Free PMC Article
    4. Curli fimbria: an Escherichia coli adhesin associated with human cystitis. Cordeiro MA, et al. Braz J Microbiol, 2016 Apr-Jun. PMID 26991275, Free PMC Article
    5. A Monte Carlo Study of the Early Steps of Functional Amyloid Formation. Tian P, et al. PLoS One, 2016. PMID 26745180, Free PMC Article

    See all (49) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A gut bacterial amyloid promotes alpha-synuclein aggregation and motor impairment in mice.
      Title: A gut bacterial amyloid promotes α-synuclein aggregation and motor impairment in mice.
    2. NMR insights into the pre-amyloid ensemble and secretion targeting of the curli subunit CsgA.
      Title: NMR insights into the pre-amyloid ensemble and secretion targeting of the curli subunit CsgA.
    3. Studies show the common structural features of CsgC and CsgE and the potential implications for general inhibition of amyloid.
      Title: Structural insights into functional amyloid inhibition in Gram -ve bacteria.
    4. CsgA deletion mutant lost its adherence to HTB-9 but continued to adhere to the HUVEC and Vero cells.
      Title: Curli fimbria: an Escherichia coli adhesin associated with human cystitis.
    5. A Monte Carlo study of the early steps of functional amyloid formation of bacterial biofilm, which is primarily formed by aggregates of the protein CsgA, has been presented.
      Title: A Monte Carlo Study of the Early Steps of Functional Amyloid Formation.
    6. Inactivation of the cysH gene results in increased surface adhesion and cell aggregation by activating the expression of the curli-encoding csgBAC operon via phosphoadenosine 59-phosphosulfate accumulation.
      Title: Sulfate assimilation pathway intermediate phosphoadenosine 59-phosphosulfate acts as a signal molecule affecting production of curli fibres in Escherichia coli.
    7. It was thus verified that the reinforcement of csgA gene in the DeltarpoS cells induced the enhanced colonization on the solid surfaces with the increased flagellum and curli expressions.
      Title: Enhanced colonization of rpoS-deficient Escherichia coli cells on solid surfaces by reinforced csgA gene expression.
    8. Substoichiometric concentrations of CsgB induce a change in the mechanism of CsgA aggregation from that of forming amorphous aggregates to that of structured intermediates similar to those of CsgB alone
      Title: The E. coli CsgB nucleator of curli assembles to β-sheet oligomers that alter the CsgA fibrillization mechanism.
    9. CsgA polymerization in vitro is preceded by the formation of thin needlelike protofibrils followed by aggregation of the amyloid fibrils.
      Title: Fibrillation of the major curli subunit CsgA under a wide range of conditions implies a robust design of aggregation.
    10. Data describe aspartic acid and glycine residues that function as "gatekeepers" to modulate CsgA polymerization efficiency and potential toxicity.
      Title: Gatekeeper residues in the major curlin subunit modulate bacterial amyloid fiber biogenesis.
    Submit: New GeneRIF Correction See all GeneRIFs (16)

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in amyloid fibril formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in cell adhesion IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of amyloid fibril formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within single-species biofilm formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in pilus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    curlin, major subunit

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1104447..1104902
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415560.1 curlin, major subunit [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415560.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      P28307
      Conserved Domains (1) summary
      PRK10051
      Location:1 → 151
      csgA; major curlin subunit CsgA
    Nucleotide Protein
    Heading Accession and Version

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