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    murJ lipid II flippase MurJ [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945487, updated on 3-Dec-2024

    Summary

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    Official Symbol
    murJ
    Official Full Name
    lipid II flippase MurJ
    Primary source
    ECOCYC:G6561
    Locus tag
    b1069
    See related
    ASAP:ABE-0003630
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1054; mviN; yceN
    Summary
    murJ(yceN) is an essental gene and conditional lethal mutants sweel and burst and acumulate the murein intermediate polyprenyl diphosphate-N-acetylmuramic acid-(pentapeptide)-N-acetyl-glucosamine at the non-permissive temperature (Inoue, 2008). murJ(yceN) was identified as one of two ORFs adjacent to the flg locus in a region originally named mviS, then renamed mviN and mviM when sequenced; mviMN was thought to be required for mouse virulence in Salmonella, but the mviMN deletion in ST39 also removed flgM (Carsiotis, 1989). flgM was later shown to be able to complement the mviS phenotype, and thus flgM is the real mouse virulence gene in this region of the Salmonella chromosome not mviS (Schmitt, 1994). [More information is available at EcoGene: EG13880]. MurJ is an essential gene involved in murein synthesis as the flippase responsible for transport of lipid II from the inner face of the inner membrane to the outer face for polymerization . [More information is available at EcoCyc: G6561].
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    Genomic context

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    Sequence:
    NC_000913.3 (1127839..1129374)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF480 domain-containing protein YceH Neighboring gene putative oxidoreductase YceM Neighboring gene flagellar biosynthesis protein FlgN Neighboring gene anti-sigma factor for FliA (sigma(28))

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The bacterial lipid II flippase MurJ functions by an alternating-access mechanism. Kumar S, et al. J Biol Chem, 2019 Jan 18. PMID 30482840, Free PMC Article
    2. FtsW activity and lipid II synthesis are required for recruitment of MurJ to midcell during cell division in Escherichia coli. Liu X, et al. Mol Microbiol, 2018 Sep. PMID 30112777
    3. Structure and mutagenic analysis of the lipid II flippase MurJ from Escherichia coli. Zheng S, et al. Proc Natl Acad Sci U S A, 2018 Jun 26. PMID 29891673, Free PMC Article
    4. Direct observation of the influence of cardiolipin and antibiotics on lipid II binding to MurJ. Bolla JR, et al. Nat Chem, 2018 Mar. PMID 29461535, Free PMC Article
    5. Charge requirements of lipid II flippase activity in Escherichia coli. Butler EK, et al. J Bacteriol, 2014 Dec. PMID 25225268, Free PMC Article

    See all (25) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Crystal structure of the lipid flippase MurJ in a ""squeezed"" form distinct from its inward- and outward-facing forms.
      Title: Crystal structure of the lipid flippase MurJ in a "squeezed" form distinct from its inward- and outward-facing forms.
    2. study provides in vivo evidence that MurJ uses an alternating-access mechanism during the lipid II transport cycle.
      Title: The bacterial lipid II flippase MurJ functions by an alternating-access mechanism.
    3. MurJ localizes both in the lateral membrane and at midcell, and is recruited to midcell simultaneously with late-localizing divisome proteins and proteins MraY and MurG. This study visualizes MurJ localization in vivo and reveals a possible mechanism of MurJ recruitment during cell division.
      Title: FtsW activity and lipid II synthesis are required for recruitment of MurJ to midcell during cell division in Escherichia coli.
    4. The crystal structure of substrate-free MurJ reveals an inward-open conformation. High-throughput mutagenesis and next-generation sequencing assessed mutational tolerance at every amino acid in the protein, providing a detailed functional and structural map for the enzyme and identifying sites for inhibitor development. A rocker-switch model for lipid II transport was supported.
      Title: Structure and mutagenic analysis of the lipid II flippase MurJ from Escherichia coli.
    5. From these data, authors hypothesize that some charged residues in the cavity region of MurJ homologs are required for interaction with lipid II and/or energy coupling during transport.
      Title: Charge requirements of lipid II flippase activity in Escherichia coli.
    6. MurJ is the lipid II flippase in Escherichia coli.
      Title: Bacterial cell wall. MurJ is the flippase of lipid-linked precursors for peptidoglycan biogenesis.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables cardiolipin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lipid-linked peptidoglycan transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables lipid-linked peptidoglycan transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lipid-linked peptidoglycan transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lipid-linked peptidoglycan transporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cell wall organization IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in glycolipid translocation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in lipid translocation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in lipid-linked peptidoglycan transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within lipid-linked peptidoglycan transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in lipid-linked peptidoglycan transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within lipid-linked peptidoglycan transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in peptidoglycan biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in peptidoglycan biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within peptidoglycan biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of cell shape IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in division septum IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    lipid II flippase MurJ

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1127839..1129374
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415587.1 lipid II flippase MurJ [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415587.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1V2G9P7, A0A2X1ND19, A0A376W583
      Conserved Domains (1) summary
      COG0728
      Location:1 → 510
      MviN; Peptidoglycan biosynthesis protein MviN/MurJ, putative lipid II flippase [Cell wall/membrane/envelope biogenesis]
    Nucleotide Protein
    Heading Accession and Version

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