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    pspA phage shock protein A [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945887, updated on 3-Dec-2024

    Summary

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    Official Symbol
    pspA
    Official Full Name
    phage shock protein A
    Primary source
    ECOCYC:EG10776
    Locus tag
    b1304
    See related
    ASAP:ABE-0004387
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    cog; ECK1299
    Summary
    A pspA deletion grows slow below 8C (as enabled by transgenic Oleispira antarctica Cpn10/60). [More information is available at EcoGene: EG10776]. PspA is a negative transcriptional regulator of the psp operon that does not directly interact with DNA . [More information is available at EcoCyc: EG10776].
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    Genomic context

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    Sequence:
    NC_000913.3 (1368079..1368747)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 4-aminobutyrate aminotransferase PuuE Neighboring gene DNA-binding transcriptional dual regulator PspF Neighboring gene phage shock protein B Neighboring gene phage shock protein C Neighboring gene phage shock protein D

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structure and function of PspA and Vipp1 N-terminal peptides: Insights into the membrane stress sensing and mitigation. McDonald C, et al. Biochim Biophys Acta Biomembr, 2017 Jan. PMID 27806910
    2. Membrane Stored Curvature Elastic Stress Modulates Recruitment of Maintenance Proteins PspA and Vipp1. McDonald C, et al. mBio, 2015 Sep 1. PMID 26330516, Free PMC Article
    3. Signal sequence processing is required for the assembly of LamB trimers in the outer membrane of Escherichia coli. Carlson JH, et al. J Bacteriol, 1993 Jun. PMID 8501036, Free PMC Article
    4. Expression of the pspA gene stimulates efficient protein export in Escherichia coli. Kleerebezem M, et al. Mol Microbiol, 1993 Mar. PMID 8387148
    5. Dissipation of proton motive force is not sufficient to induce the phage shock protein response in Escherichia coli. Engl C, et al. Curr Microbiol, 2011 May. PMID 21259006, Free PMC Article

    See all (62) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structure and function of Escherichia coli PspA and Synechocystis Vipp1 N-terminal peptides in the membrane stress sensing and mitigation have been described.
      Title: Structure and function of PspA and Vipp1 N-terminal peptides: Insights into the membrane stress sensing and mitigation.
    2. identified the minimal PspF-interacting domain of PspA, solved its structure, determined its affinity to PspF and the dissociation kinetics, identified residues that are potentially important for PspF regulation and analyzed effects of their mutation on PspF in vivo and in vitro
      Title: PspF-binding domain PspA1-144 and the PspA·F complex: New insights into the coiled-coil-dependent regulation of AAA+ proteins.
    3. Escherichia coli PspA has an enhanced sensitivity for curvature elastic (SCE) stress and a higher affinity for the membrane than Synechocystis Vipp1.
      Title: Membrane Stored Curvature Elastic Stress Modulates Recruitment of Maintenance Proteins PspA and Vipp1.
    4. The discrete spatial distribution and dynamics of PspA effector in lateral membrane regions depend on MreB and RodZ.
      Title: Anionic lipids and the cytoskeletal proteins MreB and RodZ define the spatio-temporal distribution and function of membrane stress controller PspA in Escherichia coli.
    5. By structural modelling, authors propose that PspA binds to PspF via its first two helical domains.
      Title: A key hydrophobic patch identified in an AAA⁺ protein essential for its in trans inhibitory regulation.
    6. Multiple signals are needed to induce the Psp response.
      Title: Dissipation of proton motive force is not sufficient to induce the phage shock protein response in Escherichia coli.
    7. Here the authors established that the PspA negative regulation of PspF ATPase activity is the result of a cooperative inhibition.
      Title: A lower-order oligomer form of phage shock protein A (PspA) stably associates with the hexameric AAA(+) transcription activator protein PspF for negative regulation.
    8. PspA and PspG are localized to the cell poles and traffic between both poles.
      Title: In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli.
    9. PspA can form hollow spherical or prolate spheroidal particles of about 30-40nm diameter with a scaffold-like arrangement of protein subunits at the surface.
      Title: PspA can form large scaffolds in Escherichia coli.
    10. These results indicate that oligomers of PspA bind to membrane phospholipids and suppress proton leakage.
      Title: Escherichia coli phage-shock protein A (PspA) binds to membrane phospholipids and repairs proton leakage of the damaged membranes.
    Submit: New GeneRIF Correction See all GeneRIFs (13)

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables phospholipid binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in phage shock IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within phage shock IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of cellular response to stress NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within response to heat IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cell pole IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    colocalizes_with cytoplasmic side of plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of transcription regulator complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    phage shock protein A

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1368079..1368747
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415820.1 phage shock protein A [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415820.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376M881, A0A376VK41, A0A8S7CFI5
      Conserved Domains (1) summary
      PRK10698
      Location:1 → 222
      PRK10698; phage shock protein PspA; Provisional
    Nucleotide Protein
    Heading Accession and Version

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