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    mutS DNA mismatch repair protein MutS [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947206, updated on 3-Dec-2024

    Summary

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    Official Symbol
    mutS
    Official Full Name
    DNA mismatch repair protein MutS
    Primary source
    ECOCYC:EG10625
    Locus tag
    b2733
    See related
    ASAP:ABE-0008977
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ant; ECK2728; fdv; plm
    Summary
    MutS has weak ATPase activity and may be involved in recognizing the DNA mismatch. [More information is available at EcoGene: EG10625]. MutS is one of the components of the MutHLS complex. [More information is available at EcoCyc: EG10625].
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    Genomic context

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    Sequence:
    NC_000913.3 (2857093..2859654)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional activator FhlA Neighboring gene protein YgbA Neighboring gene phosphoprotein phosphatase 2 Neighboring gene DNA-binding transcriptional repressor YgbI

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Binding specificities of the mismatch binding protein, MutS, to oligonucleotides containing modified bases. Negishi K, et al. Nucleic Acids Res Suppl, 2001. PMID 12836344
    2. Binding of mismatch repair protein MutS to mispaired DNA adducts of intercalating ruthenium(II) arene complexes. Castellano-Castillo M, et al. J Biol Inorg Chem, 2008 Aug. PMID 18491151
    3. Binding of MutS protein to oligonucleotides containing a methylated or an ethylated guanine residue, and correlation with mutation frequency. Taira K, et al. Mutat Res, 2008 Apr 2. PMID 18243250, Free PMC Article
    4. Distinct MutS DNA-binding modes that are differentially modulated by ATP binding and hydrolysis. Blackwell LJ, et al. J Biol Chem, 2001 Sep 7. PMID 11454861
    5. The Phe-X-Glu DNA binding motif of MutS. The role of hydrogen bonding in mismatch recognition. Schofield MJ, et al. J Biol Chem, 2001 Dec 7. PMID 11602569

    See all (158) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The selection process of licensing a DNA mismatch for repair.
      Title: The selection process of licensing a DNA mismatch for repair.
    2. Responses of DNA Mismatch Repair Proteins to a Stable G-Quadruplex Embedded into a DNA Duplex Structure.
      Title: Responses of DNA Mismatch Repair Proteins to a Stable G-Quadruplex Embedded into a DNA Duplex Structure.
    3. we observe that a single residue substitution (R168C) results in a high rate of head-to-tail (tandem) short sequence duplications, which are independent of existing sequence repeats. Finally, we show that the unique mutation spectrum of topA mutants enhances the emergence of antibiotic resistance in comparison to mismatch-repair (mutS) mutators, and leads to new resistance genotypes.
      Title: Point mutations in topoisomerase I alter the mutation spectrum in E. coli and impact the emergence of drug resistance genotypes.
    4. Sharp kinking of a coiled-coil in MutS allows DNA binding and release.
      Title: Sharp kinking of a coiled-coil in MutS allows DNA binding and release.
    5. Mechanism for verification of mismatched and homoduplex DNAs by nucleotides-bound MutS analyzed by molecular dynamics simulations has been reported.
      Title: Mechanism for verification of mismatched and homoduplex DNAs by nucleotides-bound MutS analyzed by molecular dynamics simulations.
    6. The assembly of an EcMutS-EcMutL-EcMutH search complex illustrates how sequential stable sliding clamps can modulate one-dimensional diffusion mechanics along the DNA to direct mismatch repair
      Title: Cascading MutS and MutL sliding clamps control DNA diffusion to activate mismatch repair.
    7. The structure captures MutS in the sliding clamp conformation, where tilting of the MutS subunits across each other pushes DNA into a new channel, and reorientation of the connector domain creates an interface for MutL with both MutS subunits.
      Title: MutS/MutL crystal structure reveals that the MutS sliding clamp loads MutL onto DNA.
    8. MutS and MutL trap recombination intermediates using mismatches formed in the heteroduplex region and secondary DNA structures in the displaced ssDNA, and recruit the UvrD helicase to resolve these trapped intermediates in a directional manner
      Title: Mismatch repair inhibits homeologous recombination via coordinated directional unwinding of trapped DNA structures.
    9. E. coli MutS has a binding activity specific for non-B form G4 DNA, but such binding appears independent of canonical heteroduplex repair activation.
      Title: G-quadruplex recognition activities of E. Coli MutS.
    10. The results confirm that specifically bound MutS bends DNA at the mismatch.
      Title: Single-molecule multiparameter fluorescence spectroscopy reveals directional MutS binding to mismatched bases in DNA.
    Submit: New GeneRIF Correction See all GeneRIFs (26)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ADP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP-dependent DNA damage sensor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding, bending IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables adenine/cytosine mispair binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables damaged DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables mismatched DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables mismatched DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables mismatched DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA damage response IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mismatch repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mismatch repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in mismatch repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mismatch repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of DNA recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of DNA recombination NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of MutS complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of mismatch repair complex IC
    Inferred by Curator
    more info
    		 PubMed 
    part_of mismatch repair complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA mismatch repair protein MutS

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2857093..2859654
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417213.1 DNA mismatch repair protein MutS [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417213.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A377C3D5, A0AAN5ZJC4
      Conserved Domains (1) summary
      PRK05399
      Location:3 → 853
      PRK05399; DNA mismatch repair protein MutS; Provisional
    Nucleotide Protein
    Heading Accession and Version

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