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    rsmE 16S rRNA m(3)U1498 methyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945816, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rsmE
    Official Full Name
    16S rRNA m(3)U1498 methyltransferase
    Primary source
    ECOCYC:EG12366
    Locus tag
    b2946
    See related
    ASAP:ABE-0009665
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2941; yggJ
    Summary
    YggJ is RsmE; although growth is not obviously impaired, wildtype outcompetes rsmE- (Basturea, 2006). [More information is available at EcoGene: EG12366]. RsmE is the methyltransferase responsible for methylation of 16S rRNA at the N3 position of the U1498 nucleotide . [More information is available at EcoCyc: EG12366].
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    Genomic context

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    Sequence:
    NC_000913.3 (3091134..3091865)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YggI Neighboring gene DNA-specific endonuclease I Neighboring gene glutathione synthetase Neighboring gene DUF179 domain-containing protein YqgE Neighboring gene ribonuclease H-like domain-containing nuclease

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Insights into the catalytic mechanism of 16S rRNA methyltransferase RsmE (m³U1498) from crystal and solution structures. Zhang H, et al. J Mol Biol, 2012 Nov 2. PMID 22925577
    2. Substrate specificity and properties of the Escherichia coli 16S rRNA methyltransferase, RsmE. Basturea GN, et al. RNA, 2007 Nov. PMID 17872509, Free PMC Article
    3. Functional assignment based on structural analysis: crystal structure of the yggJ protein (HI0303) of Haemophilus influenzae reveals an RNA methyltransferase with a deep trefoil knot. Forouhar F, et al. Proteins, 2003 Nov 1. PMID 14517985
    4. Complete nucleotide sequence of the E. coli glutathione synthetase gsh-II. Gushima H, et al. Nucleic Acids Res, 1984 Dec 21. PMID 6393055, Free PMC Article
    5. Location of the endA gene coding for endonuclease I on the physical map of the Escherichia coli K-12 chromosome. Jekel M, et al. J Bacteriol, 1994 Mar. PMID 8113204, Free PMC Article

    See all (11) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. both crystal and solution structures of E. coli RsmE were studied; study provides new information on the structure-function relationship of RsmE and thereby suggests a novel catalytic mechanism
      Title: Insights into the catalytic mechanism of 16S rRNA methyltransferase RsmE (m³U1498) from crystal and solution structures.
    2. RsmE requires a highly structured ribonucleoprotein particle as a substrate for methylation, and that methylation events in the 3' minor domain of 16S rRNA probably occur late during 30S ribosome assembly.
      Title: Substrate specificity and properties of the Escherichia coli 16S rRNA methyltransferase, RsmE.
    3. The protein encoded by yggJ, a member of the uncharacterized DUF558 protein family of predicted alpha/beta (trefoil) knot methyltransferases is responsible for methylation at U1498 in 16S rRNA.
      Title: Identification and characterization of RsmE, the founding member of a new RNA base methyltransferase family.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables rRNA (uridine-N3-)-methyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables rRNA (uridine-N3-)-methyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in rRNA base methylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within rRNA base methylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    16S rRNA m(3)U1498 methyltransferase
    NP_417421.4

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3091134..3091865
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417421.4 16S rRNA m(3)U1498 methyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417421.4

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      W1WVC8
      Conserved Domains (1) summary
      TIGR00046
      Location:2 → 242
      TIGR00046; RNA methyltransferase, RsmE family
    Nucleotide Protein
    Heading Accession and Version

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