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    tsaD N(6)-L-threonylcarbamoyladenine synthase, TsaD subunit [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947578, updated on 3-Dec-2024

    Summary

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    Official Symbol
    tsaD
    Official Full Name
    N(6)-L-threonylcarbamoyladenine synthase, TsaD subunit
    Primary source
    ECOCYC:EG11171
    Locus tag
    b3064
    See related
    ASAP:ABE-0010058
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3054; gcp; ygjD
    Summary
    The protein is essential for growth . [More information is available at EcoCyc: EG11171].
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    Genomic context

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    Sequence:
    NC_000913.3 (3209530..3210543, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene L(+)-tartrate dehydratase subunit alpha Neighboring gene L(+)-tartrate dehydratase subunit beta Neighboring gene L-tartrate:succinate antiporter Neighboring gene 30S ribosomal subunit protein S21 Neighboring gene DNA primase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Effects on transcription of mutations in ygjD, yeaZ, and yjeE genes, which are involved in a universal tRNA modification in Escherichia coli. Hashimoto C, et al. J Bacteriol, 2011 Nov. PMID 21873492, Free PMC Article
    2. Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD. Bergmiller T, et al. BMC Microbiol, 2011 May 27. PMID 21619589, Free PMC Article
    3. The ubiquitous conserved glycopeptidase Gcp prevents accumulation of toxic glycated proteins. Katz C, et al. mBio, 2010 Aug 24. PMID 20824107, Free PMC Article
    4. Evidence for non-enzymatic glycosylation in Escherichia coli. Mironova R, et al. Mol Microbiol, 2001 Feb. PMID 11251824
    5. Evidence for non-enzymatic glycosylation of Escherichia coli chromosomal DNA. Mironova R, et al. Mol Microbiol, 2005 Mar. PMID 15752201

    See all (22) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. in bacteria four proteins (YgjD, YrdC, YjeE, and YeaZ) are both necessary and sufficient for t(6)A biosynthesis in vitro
      Title: Biosynthesis of threonylcarbamoyl adenosine (t6A), a universal tRNA nucleoside.
    2. This study showed that the transcription of the thr and ilv operons in ygjD mutants was increased through the inhibition of transcription attenuation and that dnaG transcription was reduced.
      Title: Effects on transcription of mutations in ygjD, yeaZ, and yjeE genes, which are involved in a universal tRNA modification in Escherichia coli.
    3. Depletion of YgjD protein from growing cells leads to a decrease in cell size that is contingent on (p)ppGpp, and to a termination of cell division.
      Title: Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD.
    4. Escherichia coli containing a conserved Gcp prevents the accumulation of toxic glycated proteins.
      Title: The ubiquitous conserved glycopeptidase Gcp prevents accumulation of toxic glycated proteins.
    5. Repression of ygjD expression results in at least a proportion of very enlarged cells with an unusual peripheral distribution of DNA, showing that this gene is essential for the viability of E. coli.
      Title: Conserved network of proteins essential for bacterial viability.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables N(6)-L-threonylcarbamoyladenine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables acyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables acyltransferase activity, transferring groups other than amino-acyl groups IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables glycosylation-dependent protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables iron ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables iron ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in maintenance of translational fidelity NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in tRNA modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA threonylcarbamoyladenosine metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tRNA threonylcarbamoyladenosine modification IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tRNA threonylcarbamoyladenosine modification IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tRNA threonylcarbamoyladenosine modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tRNA threonylcarbamoyladenosine modification IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of EKC/KEOPS complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    N(6)-L-threonylcarbamoyladenine synthase, TsaD subunit
    NP_417536.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3209530..3210543 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417536.1 N(6)-L-threonylcarbamoyladenine synthase, TsaD subunit [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417536.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A3S4NRD4
      Conserved Domains (1) summary
      PRK09604
      Location:1 → 337
      PRK09604; tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD
    Nucleotide Protein
    Heading Accession and Version

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