dnaA chromosomal replication initiator protein DnaA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: dnaA
Official Full Name: chromosomal replication initiator protein DnaA
Primary source: ECOCYC:EG10235
Locus tag: b3702
See related: ASAP:ABE-0012103
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3694
Summary: DnaA-ATP is the active form; DnaA-ADP is inactive; switching betwee the two forms modulates initiation activity. [More information is available at EcoGene: EG10235].
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Genomic context

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Sequence:: NC_000913.3 (3882326..3883729, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mapping the Escherichia coli DnaA-binding landscape reveals a preference for binding pairs of closely spaced DNA sites.
Title: Mapping the Escherichia coli DnaA-binding landscape reveals a preference for binding pairs of closely spaced DNA sites.
Single-stranded DNA recruitment mechanism in replication origin unwinding by DnaA initiator protein and HU, an evolutionary ubiquitous nucleoid protein.
Title: Single-stranded DNA recruitment mechanism in replication origin unwinding by DnaA initiator protein and HU, an evolutionary ubiquitous nucleoid protein.
Concerted actions of DnaA complexes with DNA-unwinding sequences within and flanking replication origin oriC promote DnaB helicase loading.
Title: Concerted actions of DnaA complexes with DNA-unwinding sequences within and flanking replication origin oriC promote DnaB helicase loading.
Negative feedback for DARS2-Fis complex by ATP-DnaA supports the cell cycle-coordinated regulation for chromosome replication.
Title: Negative feedback for DARS2-Fis complex by ATP-DnaA supports the cell cycle-coordinated regulation for chromosome replication.
Topoisomerase I Essentiality, DnaA-Independent Chromosomal Replication, and Transcription-Replication Conflict in Escherichia coli.
Title: Topoisomerase I Essentiality, DnaA-Independent Chromosomal Replication, and Transcription-Replication Conflict in Escherichia coli.
Inhibition of Escherichia coli chromosome replication by rifampicin treatment or during the stringent response is overcome by de novo DnaA protein synthesis.
Title: Inhibition of Escherichia coli chromosome replication by rifampicin treatment or during the stringent response is overcome by de novo DnaA protein synthesis.
H-NS, IHF, and DnaA lead to changes in nucleoid organizations, replication initiation, and cell division.
Title: H-NS, IHF, and DnaA lead to changes in nucleoid organizations, replication initiation, and cell division.
A model for DnaA-reactivating sequences-DnaA complex dynamics during ADP dissociation; this provides novel insight into the regulatory mechanisms of DnaA and the interaction modes of AAA+ domains.
Title: A novel mode of DnaA-DnaA interaction promotes ADP dissociation for reactivation of replication initiation activity.
A nucleotide-dependent oligomerization of the Escherichia coli replication initiator DnaA requires residue His136 for remodeling of the chromosomal origin.
Title: A nucleotide-dependent oligomerization of the Escherichia coli replication initiator DnaA requires residue His136 for remodeling of the chromosomal origin.
The findings indicate both DnaA-ATP and DnaA-ADP can perform most of the mechanical functions needed for origin activation, and suggest that a key reason for ATP-regulation of DnaA is to control replication initiation frequency.
Title: Origin recognition is the predominant role for DnaA-ATP in initiation of chromosome replication.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA replication origin binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA replication origin binding	IEA Inferred from Electronic Annotation more info
enables DNA replication origin binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA replication initiation	IEA Inferred from Electronic Annotation more info
involved_in DNA unwinding involved in DNA replication	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of DNA replication	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of DNA-templated DNA replication initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated DNA replication initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA replication	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated DNA replication initiation	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of DnaA-DiaA complex	IPI Inferred from Physical Interaction more info	PubMed
part_of DnaA-Dps complex	IPI Inferred from Physical Interaction more info	PubMed
part_of DnaA-HU complex	IPI Inferred from Physical Interaction more info	PubMed
part_of DnaA-L2 complex	IPI Inferred from Physical Interaction more info	PubMed
part_of DnaA-oriC complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
part_of replication inhibiting complex	IPI Inferred from Physical Interaction more info	PubMed