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    priC primosomal replication protein N'' [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945071, updated on 17-Dec-2024

    Summary

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    Official Symbol
    priC
    Official Full Name
    primosomal replication protein N''
    Primary source
    ECOCYC:EG10765
    Locus tag
    b0467
    See related
    ASAP:ABE-0001619
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0461
    Summary
    PriC is part of the primosome, a protein complex believed to be required for restart of stalled replication forks . [More information is available at EcoCyc: EG10765].
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    Genomic context

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    Sequence:
    NC_000913.3 (490285..490812, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene potassium dependent, small conductance mechanosensitive channel Neighboring gene DUF2496 domain-containing protein YbaM Neighboring gene DUF454 domain-containing inner membrane protein YbaN Neighboring gene adenine phosphoribosyltransferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Roles of phi X174 type primosome- and G4 type primase-dependent primings in initiation of lagging and leading strand syntheses of DNA replication. Masai H, et al. J Biol Chem, 1990 Sep 5. PMID 2144283
    2. DnaT is a PriC-binding protein. Huang CC, et al. Biochem Biophys Res Commun, 2016 Sep 2. PMID 27387236
    3. PriC-mediated DNA replication restart requires PriC complex formation with the single-stranded DNA-binding protein. Wessel SR, et al. J Biol Chem, 2013 Jun 14. PMID 23629733, Free PMC Article
    4. Mutational Analysis of Residues in PriA and PriC Affecting Their Ability To Interact with SSB in Escherichia coli K-12. Klimova AN, et al. J Bacteriol, 2020 Nov 4. PMID 32900829, Free PMC Article
    5. PriA mutations that affect PriA-PriC function during replication restart. Sandler SJ, et al. Mol Microbiol, 2001 Aug. PMID 11532137

    See all (25) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Mutational Analysis of Residues in PriA and PriC Affecting Their Ability To Interact with SSB in Escherichia coli K-12.
      Title: Mutational Analysis of Residues in PriA and PriC Affecting Their Ability To Interact with SSB in Escherichia coli K-12.
    2. These results constitute a pioneering study of the DnaT-PriC interaction and present a putative link between the two independent replication restart pathways, namely, PriA- and PriC-dependent primosome assemblies. Further research can directly focus on determining how DnaT binds to the PriC-SSB-DNA tricomplex and regulates the PriC-dependent replication restart.
      Title: DnaT is a PriC-binding protein.
    3. PriC interaction with SSB remodels SSB/DNA structures at abandoned DNA replication forks to create a DNA structure that is competent for DnaB loading.
      Title: PriC-mediated DNA replication restart requires PriC complex formation with the single-stranded DNA-binding protein.
    4. N-terminus verified by Edman degradation on mature peptide
      Title: The priB and priC replication proteins of Escherichia coli. Genes, DNA sequence, overexpression, and purification.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA replication initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA replication, synthesis of primer IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication, synthesis of primer NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in DNA unwinding involved in DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within DNA-templated DNA replication IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in replication fork processing NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DnaB-DnaC-DnaT-PriA-PriC complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of DnaB-DnaC-Rep-PriC complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of primosome complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of primosome complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    primosomal replication protein N''

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      490285..490812 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415000.1 primosomal replication protein N'' [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415000.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2X1NAJ2
      Conserved Domains (1) summary
      PRK10093
      Location:5 → 175
      PRK10093; primosomal replication protein N''; Provisional
    Nucleotide Protein
    Heading Accession and Version

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