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    emrE multidrug/betaine/choline efflux transporter EmrE [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948442, updated on 3-Dec-2024

    Summary

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    Official Symbol
    emrE
    Official Full Name
    multidrug/betaine/choline efflux transporter EmrE
    Primary source
    ECOCYC:EG10629
    Locus tag
    b0543
    See related
    ASAP:ABE-0001863
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    eb; ECK0534; mvrC
    Summary
    EmrE defunct PDB structures 1S7B and 2F2M, and any conclusions derived from these structures, have been retracted due to a software data inversion error (Ma, 2007; Chang, 2007). [More information is available at EcoGene: EG10629]. EmrE is a multidrug efflux protein with a broad substrate specificity. [More information is available at EcoCyc: EG10629].
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    Genomic context

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    Sequence:
    NC_000913.3 (568315..568647)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene pseudo Neighboring gene pseudo Neighboring gene IS3 element protein InsE Neighboring gene IS3 element protein InsF Neighboring gene protein YlcJ Neighboring gene putative recombinase YbcK

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The Escherichia coli multidrug transporter EmrE is a dimer in the detergent-solubilised state. Butler PJ, et al. J Mol Biol, 2004 Jul 16. PMID 15223321
    2. Optimization of expression and the purification by organic extraction of the integral membrane protein EmrE. Winstone TL, et al. Protein Expr Purif, 2002 Oct. PMID 12356478
    3. An amino acid cluster around the essential Glu-14 is part of the substrate- and proton-binding domain of EmrE, a multidrug transporter from Escherichia coli. Gutman N, et al. J Biol Chem, 2003 May 2. PMID 12590142
    4. Structure of the substrate binding pocket of the multidrug transporter EmrE: site-directed spin labeling of transmembrane segment 1. Koteiche HA, et al. Biochemistry, 2003 May 27. PMID 12755611
    5. Examination of EmrE conformational differences in various membrane mimetic environments. Federkeil SL, et al. Biochem Cell Biol, 2003 Apr. PMID 12870870

    See all (115) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Charge neutralization of the active site glutamates does not limit substrate binding and transport by small multidrug resistance transporter EmrE.
      Title: Charge neutralization of the active site glutamates does not limit substrate binding and transport by small multidrug resistance transporter EmrE.
    2. Small multidrug resistance protein EmrE phenotypically associates with OmpW, DcrB and YggM for osmotic stress protection by betaine in Escherichia coli.
      Title: Small multidrug resistance protein EmrE phenotypically associates with OmpW, DcrB and YggM for osmotic stress protection by betaine in Escherichia coli.
    3. Activating alternative transport modes in a multidrug resistance efflux pump to confer chemical susceptibility.
      Title: Activating alternative transport modes in a multidrug resistance efflux pump to confer chemical susceptibility.
    4. Cotranslational folding and assembly of the dimeric Escherichia coli inner membrane protein EmrE.
      Title: Cotranslational folding and assembly of the dimeric Escherichia coli inner membrane protein EmrE.
    5. High-pH structure of EmrE reveals the mechanism of proton-coupled substrate transport.
      Title: High-pH structure of EmrE reveals the mechanism of proton-coupled substrate transport.
    6. Molecular mechanism of proton-coupled ligand translocation by the bacterial efflux pump EmrE.
      Title: Molecular mechanism of proton-coupled ligand translocation by the bacterial efflux pump EmrE.
    7. Inducing conformational preference of the membrane protein transporter EmrE through conservative mutations.
      Title: Inducing conformational preference of the membrane protein transporter EmrE through conservative mutations.
    8. Few conserved amino acids in the small multidrug resistance transporter EmrE influence drug polyselectivity.
      Title: Few Conserved Amino Acids in the Small Multidrug Resistance Transporter EmrE Influence Drug Polyselectivity.
    9. Optimal positions of the peptide staple were determined using free-energy simulations of peptide binding to monomeric EmrE Three of the four top-scoring peptides selected for experimental testing resulted in significant inhibition of proton-driven ethidium efflux in live cells without nonspecific toxicity. The approach described here is expected to be of general use for the design of peptide therapeutics.
      Title: Structure of the EmrE multidrug transporter and its use for inhibitor peptide design.
    10. Not only can EmrE export diverse drug substrates, it can couple antiport of a drug to either one or two protons, performing both electrogenic and electroneutral transport of a single substrate.
      Title: New free-exchange model of EmrE transport.
    Submit: New GeneRIF Correction See all GeneRIFs (39)

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables amino-acid betaine transmembrane transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables amino-acid betaine transmembrane transporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables antiporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables antiporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables antiporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables choline transmembrane transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables choline transmembrane transporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transmembrane transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables xenobiotic transmembrane transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA damage response IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cellular response to xenobiotic stimulus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in choline transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in choline transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in glycine betaine transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in glycine betaine transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to osmotic stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to xenobiotic stimulus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in xenobiotic detoxification by transmembrane export across the plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within xenobiotic metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in xenobiotic transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of EmrE multidrug transporter complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    located_in plasma membrane RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    multidrug/betaine/choline efflux transporter EmrE

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      568315..568647
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415075.1 multidrug/betaine/choline efflux transporter EmrE [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415075.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A0H3EFV7
      Conserved Domains (1) summary
      PRK09541
      Location:1 → 110
      emrE; EmrE family multidrug efflux SMR transporter
    Nucleotide Protein
    Heading Accession and Version

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