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    mukE chromosome partitioning protein MukE [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945550, updated on 3-Dec-2024

    Summary

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    Official Symbol
    mukE
    Official Full Name
    chromosome partitioning protein MukE
    Primary source
    ECOCYC:EG11252
    Locus tag
    b0923
    See related
    ASAP:ABE-0003136
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0914; kicA; ycbA
    Summary
    Condensin is required for chromosome partitioning and compaction. [More information is available at EcoGene: EG11252]. MukE plays a role in chromosome partitioning during cell division . [More information is available at EcoCyc: EG11252].
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    Genomic context

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    Sequence:
    NC_000913.3 (975622..976326)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative phosphotransferase YcbJ Neighboring gene envelope biogenesis factor Neighboring gene tRNA cmo(5)U34 methyltransferase Neighboring gene chromosome partitioning protein MukF Neighboring gene chromosome partitioning protein MukB Neighboring gene L,D-transpeptidase LdtD Neighboring gene peptidoglycan L,D-endopeptidase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Mutational analysis of MukE reveals its role in focal subcellular localization of MukBEF. She W, et al. Mol Microbiol, 2013 Feb. PMID 23171168
    2. MukE and MukF form two distinct high affinity complexes. Gloyd M, et al. J Biol Chem, 2007 May 11. PMID 17355972
    3. Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation. Prince JP, et al. Nat Commun, 2021 Nov 18. PMID 34795302, Free PMC Article
    4. The new gene mukB codes for a 177 kd protein with coiled-coil domains involved in chromosome partitioning of E. coli. Niki H, et al. EMBO J, 1991 Jan. PMID 1989883, Free PMC Article
    5. Suppression of chromosome segregation defects of Escherichia coli muk mutants by mutations in topoisomerase I. Sawitzke JA, et al. Proc Natl Acad Sci U S A, 2000 Feb 15. PMID 10660686, Free PMC Article

    See all (42) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Intersubunit and intrasubunit interactions driving the MukBEF ATPase.
      Title: Intersubunit and intrasubunit interactions driving the MukBEF ATPase.
    2. Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation.
      Title: Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation.
    3. Organization of the Escherichia coli Chromosome by a MukBEF Axial Core.
      Title: Organization of the Escherichia coli Chromosome by a MukBEF Axial Core.
    4. Complex formation requires functional interactions between the C- and N-terminal domains of MukF with the MukB head and neck, respectively, and MukE, which organizes the complexes by stabilizing binding of MukB heads to MukF. In the absence of head engagement, a MukF dimer bound by MukE forms complexes containing only a dimer of MukB.
      Title: Dynamic architecture of the Escherichia coli structural maintenance of chromosomes (SMC) complex, MukBEF.
    5. activity of MukB requires DNA binding by the head domains of the protein but does not require either ATP or its partner proteins MukE or MukF. The ability of MukB to mediate DNA catenation underscores its potential for bringing distal regions of a chromosome together.
      Title: MukB-mediated Catenation of DNA Is ATP and MukEF Independent.
    6. MatP regulates the action of topoisomerase IV and MukBEF in chromosome segregation.
      Title: MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation.
    7. Taken together, the data implicate MukBEF as a key component of the DNA segregation process by acting in concert with TopoIV to promote decatenation and positioning of newly replicated oris.
      Title: The SMC complex MukBEF recruits topoisomerase IV to the origin of replication region in live Escherichia coli.
    8. These data reveal that focal localization of MukBEF involves other activities besides DNA binding and that MukE plays a central role in them.
      Title: Mutational analysis of MukE reveals its role in focal subcellular localization of MukBEF.
    9. MukBEF complexes may undergo multiple cycles of ATP hydrolysis without being released from DNA, analogous to the behavior of well-characterized molecular motors.
      Title: In vivo architecture and action of bacterial structural maintenance of chromosome proteins.
    10. findings show here that MukBEF generates and maintains the cellular positioning of chromosome loci independently of DNA replication.
      Title: The Escherichia coli SMC complex, MukBEF, shapes nucleoid organization independently of DNA replication.
    Submit: New GeneRIF Correction See all GeneRIFs (12)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cell division IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromosome condensation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromosome condensation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in chromosome segregation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of condensin complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoid IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    chromosome partitioning protein MukE

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      975622..976326
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415443.2 chromosome partitioning protein MukE [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415443.2

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6C9QET8
      Conserved Domains (1) summary
      PRK05256
      Location:1 → 234
      PRK05256; chromosome partition protein MukE
    Nucleotide Protein
    Heading Accession and Version

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