mukB chromosome partitioning protein MukB [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: mukB
Official Full Name: chromosome partitioning protein MukB
Primary source: ECOCYC:EG10618
Locus tag: b0924
See related: ASAP:ABE-0003138
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK0915
Summary: Condensin is required for chromosome partitioning and compaction. [More information is available at EcoGene: EG10618]. The MukB protein is involved in chromosome condensation and partitioning. [More information is available at EcoCyc: EG10618].
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Genomic context

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Sequence:: NC_000913.3 (976326..980786)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Intersubunit and intrasubunit interactions driving the MukBEF ATPase.
Title: Intersubunit and intrasubunit interactions driving the MukBEF ATPase.
Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation.
Title: Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation.
Organization of the Escherichia coli Chromosome by a MukBEF Axial Core.
Title: Organization of the Escherichia coli Chromosome by a MukBEF Axial Core.
Complex formation requires functional interactions between the C- and N-terminal domains of MukF with the MukB head and neck, respectively, and MukE, which organizes the complexes by stabilizing binding of MukB heads to MukF. In the absence of head engagement, a MukF dimer bound by MukE forms complexes containing only a dimer of MukB.
Title: Dynamic architecture of the Escherichia coli structural maintenance of chromosomes (SMC) complex, MukBEF.
The authors show that a 4-helix bundle adjacent to the MukF dimerisation domain interacts functionally with the MukB coiled-coiled 'neck' adjacent to the ATPase head. They propose that this interaction leads to an asymmetric tripartite complex, as in other SMC (Structural Maintenance of Chromosomes) complexes.
Title: MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin.
Study reports that MukB binds topologically to DNA in vitro, and this binding is preferentially single-stranded DNA (ssDNA) rather than double-stranded DNA. The binding of MukB to ssDNA does not require ATP but the thermal energy enhances the binding.
Title: In vitro topological loading of bacterial condensin MukB on DNA, preferentially single-stranded DNA rather than double-stranded DNA.
MukB hinge region binds DNA and that this region of the protein is involved in sequestration of supercoils. Cells carrying mutations in the MukB hinge that reduce DNA condensation in vitro exhibit nucleoid decondensation in vivo.
Title: The bacterial condensin MukB compacts DNA by sequestering supercoils and stabilizing topologically isolated loops.
Cells carrying a mukB mutant allele that encodes a protein that does not interact with topoisomerase IV exhibit severe nucleoid decompaction leading to chromosome segregation defects. These findings suggest that the MukB-topoisomerase IV complex may provide a scaffold for DNA condensation.
Title: The MukB-topoisomerase IV interaction is required for proper chromosome compaction.
activity of MukB requires DNA binding by the head domains of the protein but does not require either ATP or its partner proteins MukE or MukF. The ability of MukB to mediate DNA catenation underscores its potential for bringing distal regions of a chromosome together.
Title: MukB-mediated Catenation of DNA Is ATP and MukEF Independent.
MatP regulates the action of topoisomerase IV and MukBEF in chromosome segregation.
Title: MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables GTP binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromosome condensation	IDA Inferred from Direct Assay more info	PubMed
involved_in chromosome condensation	IEA Inferred from Electronic Annotation more info
involved_in chromosome condensation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromosome condensation	NAS Non-traceable Author Statement more info	PubMed
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of condensin complex	IDA Inferred from Direct Assay more info	PubMed
part_of condensin complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoid	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: chromosome partitioning protein MukB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

976326..980786

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_415444.1 chromosome partitioning protein MukB [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_415444.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A376FNT7, A0A793J1H8, A0A8S7TEG9

Conserved Domains (1) summary

COG3096
Location:1 → 1480

MukB; Chromosome condensin MukBEF, ATPase and DNA-binding subunit MukB [Escherichia coli str. K-12 substr. MG1655 [Cell cycle control, cell division, chromosome partitioning]