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    mlc DNA-binding transcriptional repressor Mlc [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945510, updated on 3-Dec-2024

    Summary

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    Official Symbol
    mlc
    Official Full Name
    DNA-binding transcriptional repressor Mlc
    Primary source
    ECOCYC:G6852
    Locus tag
    b1594
    See related
    ASAP:ABE-0005323
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    dgsA; ECK1589
    Summary
    DgsA, better known as Mlc, "makes large colonies," is a transcriptional dual regulator that controls the expression of a number of genes encoding enzymes of the Escherichia coli phosphotransferase (PTS) and phosphoenolpyruvate (PEP) systems . [More information is available at EcoCyc: G6852].
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    Genomic context

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    Sequence:
    NC_000913.3 (1667344..1668564, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene redox enzyme maturation protein DmsD Neighboring gene putative chloride:H(+) antiporter ClcB Neighboring gene dethiobiotin synthetase BidA Neighboring gene putative DNA-binding transcriptional regulator YnfL Neighboring gene putative transporter YnfM

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Dual inducer signal recognition by an Mlc homologue. Bréchemier-Baey D, et al. Microbiology (Reading), 2015 Aug. PMID 26293172
    2. Transcription regulators controlled by interaction with enzyme IIB components of the phosphoenolpyruvate: sugar phosphotransferase system. Joyet P, et al. Biochim Biophys Acta, 2013 Jul. PMID 23318733
    3. Decreasing accumulation of acetate in a rich medium by Escherichia coli on introduction of genes on a multicopy plasmid. Hosono K, et al. Biosci Biotechnol Biochem, 1995 Feb. PMID 7766024
    4. Regulation of gene expression in the PTS in Escherichia coli: the role and interactions of Mlc. Plumbridge J. Curr Opin Microbiol, 2002 Apr. PMID 11934616
    5. Crystallization and preliminary X-ray analysis of Mlc from Escherichia coli. Gerber K, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2005 Feb 1. PMID 16510988, Free PMC Article

    See all (49) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Mlc can be sequestered by both PtsG and NagE but that sequestration by NagE is only significant when the NagE level is high.
      Title: Dual inducer signal recognition by an Mlc homologue.
    2. This work identifies four amino acids in the linker region which allow Mlc to distinguish between a C/G and an A/T bp at positions +/-11 of the operators.
      Title: Operator recognition by the ROK transcription factor family members, NagC and Mlc.
    3. Studies indicate tht control mechanism of the repressor Mlc by interaction with enzyme IIB components of the phosphoenolpyruvate: sugar phosphotransferase system.
      Title: Transcription regulators controlled by interaction with enzyme IIB components of the phosphoenolpyruvate: sugar phosphotransferase system.
    4. Based on these findings, the authors propose a model of MtfA as a glucose-regulated peptidase activated by cytoplasmic Mlc.
      Title: Characterization of MtfA, a novel regulatory output signal protein of the glucose-phosphotransferase system in Escherichia coli K-12.
    5. Results show that regulation of ptsG by Mlc involves some cooperative binding of Mlc to the two Mlc operators upstream of ptsG.
      Title: Switching control of expression of ptsG from the Mlc regulon to the NagC regulon.
    6. The crystal structure of a tetrameric Mlc in a complex with four molecules of enzyme IIB(Glc) (EIIB), the cytoplasmic domain of EIICB(Glc), is reported.
      Title: Analyses of Mlc-IIBGlc interaction and a plausible molecular mechanism of Mlc inactivation by membrane sequestration.
    7. Repression by chimeric proteins Mlc confirms the importance of the C-terminal region of Mlc for both repression and inducer binding and show that the helix-turn-helix DNA-binding motif is not sufficient to interact with DNA.
      Title: Different regions of Mlc and NagC, homologous transcriptional repressors controlling expression of the glucose and N-acetylglucosamine phosphotransferase systems in Escherichia coli, are required for inducer signal recognition.
    8. crystal structure of Mlc, a global regulator of sugar metabolism
      Title: The crystal structure of Mlc, a global regulator of sugar metabolism in Escherichia coli.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA-templated transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasm TAS
    Traceable Author Statement
    more info
    		 PubMed 
    located_in membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA-binding transcriptional repressor Mlc

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1667344..1668564 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416111.1 DNA-binding transcriptional repressor Mlc [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416111.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A377DS98
      Conserved Domains (2) summary
      COG1940
      Location:80 → 406
      NagC; Sugar kinase of the NBD/HSP70 family, may contain an N-terminal HTH domain [Transcription, Carbohydrate transport and metabolism]
      cl21459
      Location:20 → 60
      HTH; Helix-turn-helix domains
    Nucleotide Protein
    Heading Accession and Version

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