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    lpxT lipid A-core phosphotransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946693, updated on 3-Dec-2024

    Summary

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    Official Symbol
    lpxT
    Official Full Name
    lipid A-core phosphotransferase
    Primary source
    ECOCYC:G7146
    Locus tag
    b2174
    See related
    ASAP:ABE-0007196
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2168; yeiU
    Summary
    One third of the lipid A pool is lipid A 1-diphosphate formed by a periplasmic domain of LpxT (Touze, 2008). ybjG, pgpB, and lpxT are paralogs. [More information is available at EcoGene: EG14075]. LpxT is responsible for transfer of a phosphate group from undecaprenyl-pyrophosphate to the position 1 phosphate of approximately one-third of lipid A molecules to create lipid A 1-diphosphate. [More information is available at EcoCyc: G7146].
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    Genomic context

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    Sequence:
    NC_000913.3 (2268854..2269567)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative oxidoreductase YeiQ Neighboring gene zinc-binding GTPase YeiR Neighboring gene peptidoglycan endopeptidase/peptidoglycan L,D-carboxypeptidase Neighboring gene putative c-di-GMP phosphodiesterase PdeN

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Temperature-dependent regulation of the Escherichia coli lpxT gene. Sciandrone B, et al. Biochim Biophys Acta Gene Regul Mech, 2019 Aug. PMID 31299227
    2. Activation of PmrA inhibits LpxT-dependent phosphorylation of lipid A promoting resistance to antimicrobial peptides. Herrera CM, et al. Mol Microbiol, 2010 Jun. PMID 20384697, Free PMC Article
    3. Undecaprenyl phosphate recycling comes out of age. Valvano MA. Mol Microbiol, 2008 Jan. PMID 18086187
    4. LpxT-Dependent Phosphorylation of Lipid A in Escherichia coli Increases Resistance to Deoxycholate and Enhances Gut Colonization. Tian X, et al. Front Microbiol, 2021. PMID 34017319, Free PMC Article
    5. Periplasmic phosphorylation of lipid A is linked to the synthesis of undecaprenyl phosphate. Touzé T, et al. Mol Microbiol, 2008 Jan. PMID 18047581, Free PMC Article

    See all (18) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Results show that the Escherichia coli lpxT gene is down-regulated upon temperature upshift and found that the protein stability is not affected by the temperature change. Conversely, the lpxT mRNA levels strongly decrease upon a shift from 28 to 42 degrees C. The lpxT promoter has a temperature-sensitive activity depending on its peculiar -10 region.
      Title: Temperature-dependent regulation of the Escherichia coli lpxT gene.
    2. PmrA-dependent inhibition of LpxT is required for phosphoethanolamine decoration of lipid A, which is shown here to be critical for Escherichia coli to resist the bactericidal activity of polymyxin.
      Title: Activation of PmrA inhibits LpxT-dependent phosphorylation of lipid A promoting resistance to antimicrobial peptides.
    3. Together, the results support the notion that YbjG, and to a lesser extent YeiU, exert their enzymic activity on the periplasmic side of the plasma membrane and are implicated in the recycling of periplasmic undecaprenyl pyrophosphate molecules.
      Title: An Escherichia coli undecaprenyl-pyrophosphate phosphatase implicated in undecaprenyl phosphate recycling.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables phosphotransferase activity, phosphate group as acceptor IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphotransferase activity, phosphate group as acceptor IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables undecaprenyl-diphosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in Gram-negative-bacterium-type cell outer membrane assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lipid A biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lipopolysaccharide biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to bile acid IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    lipid A-core phosphotransferase
    NP_416679.4

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2268854..2269567
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416679.4 lipid A-core phosphotransferase [Escherichia coli str. K-12 substr. MG1655]

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376SBR1, A0A418GGF0, A0A8S7HXC4
      Conserved Domains (1) summary
      smart00014
      Location:98 → 210
      acidPPc; Acid phosphatase homologues
    Nucleotide Protein
    Heading Accession and Version

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