U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    barA sensor histidine kinase BarA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947255, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    barA
    Official Full Name
    sensor histidine kinase BarA
    Primary source
    ECOCYC:EG11367
    Locus tag
    b2786
    See related
    ASAP:ABE-0009131
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    airS; ECK2780
    Summary
    BarA has both sensory kinase and response regulator domains. [More information is available at EcoGene: EG11367]. The BarA protein contains both a sensory kinase and a response regulator domain as well as a second phosphorylated histidine site in the C-terminal region . [More information is available at EcoCyc: EG11367].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (2915057..2917813)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene GDP/GTP pyrophosphokinase Neighboring gene 23S rRNA m(5)U1939 methyltransferase Neighboring gene D-glucarate dehydratase Neighboring gene glucarate dehydratase-related protein

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. The Escherichia coli two-component signal sensor BarA binds protonated acetate via a conserved hydrophobic-binding pocket. Alvarez AF, et al. J Biol Chem, 2021 Dec. PMID 34743001, Free PMC Article
    2. A novel sensor-regulator protein that belongs to the homologous family of signal-transduction proteins involved in adaptive responses in Escherichia coli. Nagasawa S, et al. Mol Microbiol, 1992 Mar. PMID 1574005
    3. The physiological stimulus for the BarA sensor kinase. Chavez RG, et al. J Bacteriol, 2010 Apr. PMID 20118252, Free PMC Article
    4. The bacterial adaptive response gene, barA, encodes a novel conserved histidine kinase regulatory switch for adaptation and modulation of metabolism in Escherichia coli. Sahu SN, et al. Mol Cell Biochem, 2003 Nov. PMID 14619967
    5. A Novel Role of the Two-Component System Response Regulator UvrY in Enterohemorrhagic Escherichia coli O157:H7 Pathogenicity Regulation. Wu P, et al. Int J Mol Sci, 2023 Jan 24. PMID 36768620, Free PMC Article

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Two Polyketides Intertwined in Complex Regulation: Posttranscriptional CsrA-Mediated Control of Colibactin and Yersiniabactin Synthesis in Escherichia coli.
      Title: Two Polyketides Intertwined in Complex Regulation: Posttranscriptional CsrA-Mediated Control of Colibactin and Yersiniabactin Synthesis in Escherichia coli.
    2. A Novel Role of the Two-Component System Response Regulator UvrY in Enterohemorrhagic Escherichia coli O157:H7 Pathogenicity Regulation.
      Title: A Novel Role of the Two-Component System Response Regulator UvrY in Enterohemorrhagic Escherichia coli O157:H7 Pathogenicity Regulation.
    3. The Escherichia coli two-component signal sensor BarA binds protonated acetate via a conserved hydrophobic-binding pocket.
      Title: The Escherichia coli two-component signal sensor BarA binds protonated acetate via a conserved hydrophobic-binding pocket.
    4. CsrA is required for properly switching BarA from its phosphatase to its kinase activity.
      Title: Effects of the global regulator CsrA on the BarA/UvrY two-component signaling system.
    5. The authors show that the metabolic end products formate and acetate provide a physiological stimulus for the BarA/UvrY two-component signal transduction system activating the transcription of CsrB and CsrC noncoding RNAs.
      Title: The physiological stimulus for the BarA sensor kinase.
    6. genetic characterization of the BarA-UvrY two-component system by complementation; site-directed mutagenesis of HAMP linker-mutated protein demonstrated that the phosphoaccepting aspartate 718 and histidine 861 are crucial for dephosphorylating activity
      Title: Genetic and functional characterization of the Escherichia coli BarA-UvrY two-component system: point mutations in the HAMP linker of the BarA sensor give a dominant-negative phenotype.
    7. The role of Escherichia coli BarA-UvrY two-component system in virulence using a monkey cystitis model is reported.
      Title: The Escherichia coli BarA-UvrY two-component system is a virulence determinant in the urinary tract.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables histidine phosphotransfer kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphoprotein phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphorelay sensor kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphorelay sensor kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein histidine kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in phosphorelay signal transduction system IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to acetate IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to formic acid IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to hydrogen peroxide IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within signal transduction EXP
    Inferred from Experiment
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    sensor histidine kinase BarA
    NP_417266.1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2915057..2917813
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417266.1 sensor histidine kinase BarA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417266.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6C9Q766, A0A793CGN7
      Conserved Domains (1) summary
      PRK11107
      Location:1 → 918
      PRK11107; hybrid sensory histidine kinase BarA; Provisional
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.