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    adk adenylate kinase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945097, updated on 3-Dec-2024

    Summary

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    Official Symbol
    adk
    Official Full Name
    adenylate kinase
    Primary source
    ECOCYC:EG10032
    Locus tag
    b0474
    See related
    ASAP:ABE-0001645
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    dnaW; ECK0468; plsA
    Summary
    Adenylate kinase is an essential enzyme required for the biosynthesis of purine ribonucleotides and has a key role in controlling the rate of cell growth . [More information is available at EcoCyc: EG10032].
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    Genomic context

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    Sequence:
    NC_000913.3 (497175..497819)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative nucleoid-associated protein YbaB Neighboring gene recombination mediator protein RecR Neighboring gene chaperone protein HtpG Neighboring gene ferrochelatase Neighboring gene acetylesterase Neighboring gene inosine/guanosine kinase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Study of global motions in proteins by weighted masses molecular dynamics: adenylate kinase as a test case. Elamrani S, et al. Proteins, 1996 May. PMID 8727320
    2. Genetic analysis of Escherichia coli mutants defective in adenylate kinase and sn-glycerol 3-phosphate acyltransferase. Esmon BE, et al. J Bacteriol, 1980 Jan. PMID 6243627, Free PMC Article
    3. Adenylate energy charge in Escherichia coli CR341T28 and properties of heat-sensitive adenylate kinase. Glembotski CC, et al. J Bacteriol, 1981 Mar. PMID 6259132, Free PMC Article
    4. Adenylate kinase of Escherichia coli: evidence for a functional interaction in phospholipid synthesis. Goelz SE, et al. Biochemistry, 1982 Jan 5. PMID 6277367
    5. The Escherichia coli dnaW mutation is an allele of the adk gene. Henson JM, et al. Mol Gen Genet, 1982. PMID 6290847

    See all (108) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Adenylate kinase (ADK) conformations segregate naturally into open; intermediate; and closed forms with long-term residence in the intermediate state.
      Title: Fine structure of conformational ensembles in adenylate kinase.
    2. These results are consistent with an induced-fit model in which the transition of AdK from an open state to a closed state is initiated by ATP binding.
      Title: Effects of Catalytic Action and Ligand Binding on Conformational Ensembles of Adenylate Kinase.
    3. Fast closure of N-terminal long loops but slow formation of beta strands precedes the folding transition state of Escherichia coli adenylate kinase.
      Title: Fast closure of N-terminal long loops but slow formation of β strands precedes the folding transition state of Escherichia coli adenylate kinase.
    4. As both open and closed states sampled a wide range of conformation transitions, our simulation strongly supported the conformational selection mechanism for Escherichia coli ADK.
      Title: Molecular dynamics studies on the conformational transitions of adenylate kinase: a computational evidence for the conformational selection mechanism.
    5. fast subdomain folding prior to global refolding transition of adenylate kinase; applied double kinetics method to test the hypothesis that specific short native-like intramolecular distances between residues separated by long chain segments in a globular protein can be formed in the collapsed state, prior to the cooperative folding transition
      Title: Fast subdomain folding prior to the global refolding transition of E. coli adenylate kinase: a double kinetics study.
    6. In Cartesian space, LID domain closure precedes NMP domain closure in the bound simulation, consistent with prior coarse-grained models of the adenylate kinase conformational transition.
      Title: Many local motions cooperate to produce the adenylate kinase conformational transition.
    7. Results demonstrate that the conformational ensemble of adenylate kinase is significantly populated by a locally unfolded state and that the excited-state can be manipulated through mutation, independent of perturbations of the ground-state structures.
      Title: Rational modulation of conformational fluctuations in adenylate kinase reveals a local unfolding mechanism for allostery and functional adaptation in proteins.
    8. Results show that the flexible subdomains of adk fold into their native structure in a noncooperative manner with respect to the CORE subdomain.
      Title: Noncooperative folding of subdomains in adenylate kinase.
    9. Results describe the atomistic mechanism of conformational transition in adenylate kinase.
      Title: The atomistic mechanism of conformational transition in adenylate kinase: a TEE-REX molecular dynamics study.
    10. N-terminus verified by Edman degradation on complete protein
      Title: Protein identification with N and C-terminal sequence tags in proteome projects.
    Submit: New GeneRIF Correction See all GeneRIFs (16)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables AMP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables adenylate kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables adenylate kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables adenylate kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables adenylate kinase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nucleoside diphosphate kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables nucleoside diphosphate kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nucleoside monophosphate kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within ADP biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in AMP salvage IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in adenine metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in nucleobase-containing compound metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in nucleoside diphosphate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in nucleoside monophosphate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in nucleoside monophosphate phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in nucleotide biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within purine ribonucleotide interconversion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    adenylate kinase
    NP_415007.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      497175..497819
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415007.1 adenylate kinase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415007.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A0J9X1X4
      Conserved Domains (1) summary
      PRK00279
      Location:1 → 214
      adk; adenylate kinase; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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