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    cheY chemotaxis protein CheY [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946393, updated on 3-Dec-2024

    Summary

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    Official Symbol
    cheY
    Official Full Name
    chemotaxis protein CheY
    Primary source
    ECOCYC:EG10150
    Locus tag
    b1882
    See related
    ASAP:ABE-0006280
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1883
    Summary
    CheY is acetylated at lysine residues 92 and 109 by acetyl-CoA synthase (Acs). [More information is available at EcoGene: EG10150]. Chemotaxis regulator protein CheY is activated through phosphorylation of an aspartate residue by the CheA(L)-his-P protein. [More information is available at EcoCyc: EG10150].
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    Genomic context

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    Sequence:
    NC_000913.3 (1967048..1967437, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene flagellar biosynthesis protein FlhA Neighboring gene flagellar biosynthesis protein FlhB Neighboring gene chemotaxis protein CheZ Neighboring gene protein-glutamate methylesterase/protein glutamine deamidase Neighboring gene chemotaxis protein methyltransferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Tyrosine 106 of CheY plays an important role in chemotaxis signal transduction in Escherichia coli. Zhu X, et al. J Bacteriol, 1996 Jul. PMID 8763950, Free PMC Article
    2. The nonequilibrium mechanism for ultrasensitivity in a biological switch: sensing by Maxwell's demons. Tu Y. Proc Natl Acad Sci U S A, 2008 Aug 19. PMID 18687900, Free PMC Article
    3. Kinetic traps in the folding of beta alpha-repeat proteins: CheY initially misfolds before accessing the native conformation. Kathuria SV, et al. J Mol Biol, 2008 Oct 3. PMID 18619461
    4. Two variable active site residues modulate response regulator phosphoryl group stability. Thomas SA, et al. Mol Microbiol, 2008 Jul. PMID 18557815, Free PMC Article
    5. Interactions between chemotaxis genes and flagellar genes in Escherichia coli. Parkinson JS, et al. J Bacteriol, 1983 Jul. PMID 6305913, Free PMC Article

    See all (161) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility.
      Title: The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility.
    2. Mechanosensitive recruitment of stator units promotes binding of the response regulator CheY-P to the flagellar motor.
      Title: Mechanosensitive recruitment of stator units promotes binding of the response regulator CheY-P to the flagellar motor.
    3. Fluctuations in Intracellular CheY-P Concentration Coordinate Reversals of Flagellar Motors in E. coli.
      Title: Fluctuations in Intracellular CheY-P Concentration Coordinate Reversals of Flagellar Motors in E. coli.
    4. Modulation of Response Regulator CheY Reaction Kinetics by Two Variable Residues That Affect Conformation.
      Title: Modulation of Response Regulator CheY Reaction Kinetics by Two Variable Residues That Affect Conformation.
    5. The suitability for FRET analysis as well as functionality of fusions with SNAP- and HaloTag could be demonstrated by studying the transient interaction between chemotaxis response regulator CheY and its phosphatase CheZ
      Title: A gateway-based system for fast evaluation of protein-protein interactions in bacteria.
    6. study identified K91 and K109 as the major sites whose acetylation level in vivo increases in response to acetate
      Title: CheY's acetylation sites responsible for generating clockwise flagellar rotation in Escherichia coli.
    7. the enhanced reactivity of CheY DR reflected partial acquisition of catalytic and structural features of HAD phosphatases.
      Title: Probing Mechanistic Similarities between Response Regulator Signaling Proteins and Haloacid Dehalogenase Phosphatases.
    8. Authors observe an apparent decrease and redistribution of mus-ms dynamics of allosteric response upon phosphorylation (and accompanying Mg(2+) saturation) of CheY.
      Title: Colocalization of fast and slow timescale dynamics in the allosteric signaling protein CheY.
    9. The flagellar motor adapts to changes in steady-state level of chemotaxis response regulator CheY-P by adjusting the number of FliM molecules to which CheY-P binds. Extreme motor ultrasensitivity broadens our understanding of allostery mechanisms
      Title: Ultrasensitivity of an adaptive bacterial motor.
    10. Amino acid substitutions distant from the active site can affect CheZ catalytic activity and CheYp binding, perhaps via the propagation of structural or dynamic perturbations through a helical bundle.
      Title: Action at a distance: amino acid substitutions that affect binding of the phosphorylated CheY response regulator and catalysis of dephosphorylation can be far from the CheZ phosphatase active site.
    Submit: New GeneRIF Correction See all GeneRIFs (23)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables acetyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphorelay response regulator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within aerotaxis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in archaeal or bacterial-type flagellum-dependent cell motility IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in bacterial-type flagellum-dependent swimming motility IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in bacterial-type flagellum-dependent swimming motility NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in chemotaxis IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within chemotaxis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in chemotaxis NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in internal peptidyl-lysine acetylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in phosphorelay signal transduction system IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in phosphorelay signal transduction system IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of bacterial-type flagellum-dependent cell motility IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of bacterial-type flagellum-dependent cell motility IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of bacterial-type flagellum-dependent cell motility IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of bacterial-type flagellum-dependent cell motility NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in regulation of chemotaxis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of chemotaxis NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within signal transduction IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within thermotaxis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in bacterial-type flagellum NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in bacterial-type flagellum basal body, C ring NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in bacterial-type flagellum rotor complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    chemotaxis protein CheY

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1967048..1967437 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416396.1 chemotaxis protein CheY [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416396.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AJ61
      Conserved Domains (1) summary
      PRK10610
      Location:1 → 129
      PRK10610; chemotaxis protein CheY
    Nucleotide Protein
    Heading Accession and Version

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