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    rsuA 16S rRNA pseudouridine(516) synthase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945378, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rsuA
    Official Full Name
    16S rRNA pseudouridine(516) synthase
    Primary source
    ECOCYC:EG12044
    Locus tag
    b2183
    See related
    ASAP:ABE-0007223
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2177; yejD
    Summary
    RsuA is a survival protein required for MazF-mediated survival of a small subpopulation after inhibition of translation (Amitai, 2009). [More information is available at EcoGene: EG12044]. RsuA is the pseudouridine synthase that is responsible for pseudouridylation of 16S rRNA at position 516 . [More information is available at EcoCyc: EG12044].
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    Genomic context

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    Sequence:
    NC_000913.3 (2279788..2280483, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YejG Neighboring gene multidrug efflux pump Bcr Neighboring gene putative DNA repair helicase RadD Neighboring gene 50S ribosomal subunit protein L25

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Pseudouridine Synthase RsuA Captures an Assembly Intermediate that Is Stabilized by Ribosomal Protein S17. Jayalath K, et al. Biomolecules, 2020 May 30. PMID 32486254, Free PMC Article
    2. Insights into the biology of Escherichia coli through structural proteomics. Matte A, et al. J Struct Funct Genomics, 2007 Sep. PMID 17668295
    3. Purification, cloning, and properties of the 16S RNA pseudouridine 516 synthase from Escherichia coli. Wrzesinski J, et al. Biochemistry, 1995 Jul 11. PMID 7612632
    4. Life, death, differentiation, and the multicellularity of bacteria. Rosenberg SM. PLoS Genet, 2009 Mar. PMID 19282973, Free PMC Article
    5. Escherichia coli MazF leads to the simultaneous selective synthesis of both "death proteins" and "survival proteins". Amitai S, et al. PLoS Genet, 2009 Mar. PMID 19282968, Free PMC Article

    See all (15) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Pseudouridine Synthase RsuA Captures an Assembly Intermediate that Is Stabilized by Ribosomal Protein S17.
      Title: Pseudouridine Synthase RsuA Captures an Assembly Intermediate that Is Stabilized by Ribosomal Protein S17.
    2. N-terminus verified by Edman degradation on complete protein
      Title: Purification, cloning, and properties of the 16S RNA pseudouridine 516 synthase from Escherichia coli.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 16S rRNA pseudouridine(516) synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables pseudouridine synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables pseudouridine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables rRNA pseudouridine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables rRNA pseudouridine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in enzyme-directed rRNA pseudouridine synthesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in enzyme-directed rRNA pseudouridine synthesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in enzyme-directed rRNA pseudouridine synthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in pseudouridine synthesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in rRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    16S rRNA pseudouridine(516) synthase
    NP_416688.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2279788..2280483 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416688.1 16S rRNA pseudouridine(516) synthase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416688.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6C9QHK6
      Conserved Domains (1) summary
      PRK10839
      Location:1 → 230
      PRK10839; 16S rRNA pseudouridine(516) synthase RsuA
    Nucleotide Protein
    Heading Accession and Version

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