ptsI PTS enzyme I [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: ptsI
Official Full Name: PTS enzyme I
Primary source: ECOCYC:EG10789
Locus tag: b2416
See related: ASAP:ABE-0007967
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ctr; ECK2411
Summary: Enzyme I and HPr are the two sugar-non-specific protein constituents of the PTS. [More information is available at EcoCyc: EG10789].
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Genomic context

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Sequence:: NC_000913.3 (2534066..2535793)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

When the oligomerization state of EI is shifted toward the dimeric species, alpha-ketoglutarate functions as a competitive inhibitor of EI.
Title: The oligomerization state of bacterial enzyme I (EI) determines EI's allosteric stimulation or competitive inhibition by α-ketoglutarate.
Study elucidated the structure of the NPr:EIN Ntr complex using a hybrid approach. The structure has a similar fold to the homologous HPr:EIN sugar complex, but differs in surface potential, which explains the difference in specificity between the two evolutionarily related enzyme pairs.
Title: Structure of the NPr:EIN(Ntr) Complex: Mechanism for Specificity in Paralogous Phosphotransferase Systems.
Isolated a stable recombinant C-terminal domain of Enzyme I (EIC) of Escherichia coli and characterized its interaction with the N-terminal domain of Enzyme I (EIN) and also with various ligands.
Title: Calorimetric and spectroscopic investigation of the interaction between the C-terminal domain of Enzyme I and its ligands.
N-terminus verified by Edman degradation on complete protein
Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
crystal structure reveals a dimeric protein where each subunit comprises three domains
Title: Structure of phosphorylated enzyme I, the phosphoenolpyruvate:sugar phosphotransferase system sugar translocation signal protein.
EI and EI-H189Q each comprise a multiplicity of conformers and progressively fewer conformers as they dimerize and bind Mg(2+) and finally PEP
Title: The monomer/dimer transition of enzyme I of the Escherichia coli phosphotransferase system.
the phosphotransfer rate increases approximately 25-fold when HPr is added to a mixture of EI-N, EI-C, and PEP(Mg(2+))
Title: Properties of the C-terminal domain of enzyme I of the Escherichia coli phosphotransferase system.
Subcellular distribution depends on growth conditions
Title: Subcellular distribution of enzyme I of the Escherichia coli phosphoenolpyruvate:glycose phosphotransferase system depends on growth conditions.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphoenolpyruvate-protein phosphotransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphoenolpyruvate-protein phosphotransferase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in N-acetylglucosamine transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphoenolpyruvate-dependent sugar phosphotransferase system	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed

General protein information

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Preferred Names: PTS enzyme I

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

2534066..2535793

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_416911.1 PTS enzyme I [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_416911.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A376J2V9, A0A376M4Z9

Conserved Domains (1) summary

PRK11177
Location:1 → 575

PRK11177; phosphoenolpyruvate-protein phosphotransferase PtsI