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    guaB inosine 5'-monophosphate dehydrogenase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946985, updated on 3-Dec-2024

    Summary

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    Official Symbol
    guaB
    Official Full Name
    inosine 5'-monophosphate dehydrogenase
    Primary source
    ECOCYC:EG10421
    Locus tag
    b2508
    See related
    ASAP:ABE-0008257
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2504; guaR
    Summary
    De novo biosyntheis of guanine nucleotides is rate-limited by IMP. [More information is available at EcoGene: EG10421]. IMP dehydrogenase (IMPDH) is a homotetramer . [More information is available at EcoCyc: EG10421].
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    Genomic context

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    Sequence:
    NC_000913.3 (2632604..2634070, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene nalidixic acid resistance protein YfgI Neighboring gene GMP synthetase Neighboring gene exodeoxyribonuclease VII subunit XseA Neighboring gene zinc ribbon domain-containing protein YfgJ Neighboring gene 50S ribosomal subunit stability factor

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Asp338 controls hydride transfer in Escherichia coli IMP dehydrogenase. Kerr KM, et al. Biochemistry, 2000 Aug 15. PMID 10933797
    2. Complementation in vitro between guaB mutants of Escherichia coli K12. Gilbert HJ, et al. J Gen Microbiol, 1980 Mar. PMID 6104688
    3. Active-site modification of native and mutant forms of inosine 5'-monophosphate dehydrogenase from Escherichia coli K12. Gilbert HJ, et al. Biochem J, 1980 Nov 1. PMID 6112982, Free PMC Article
    4. Dual control of the gua operon of Escherichia coli K12 by adenine and guanine nucleotides. Mehra RK, et al. J Gen Microbiol, 1981 Mar. PMID 6119351
    5. Construction and characterization of guaB-lacZ fusions in Escherichia coli K12. Spibey N, et al. J Gen Microbiol, 1981 Oct. PMID 6461728

    See all (59) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data show that the CRP-cyclic AMP (cAMP) complex binds to a site located over 100 bp upstream of the guaB transcription start site, where it serves to downregulate P(guaB).
      Title: Downregulation of the Escherichia coli guaB promoter by upstream-bound cyclic AMP receptor protein.
    2. Binding of FIS to binding sites centred near positions -11, +8 and +29 relative to the guaB transcription start site results in repression of guaB promoter in vitro but not in vivo, and deletion of the fis gene increased guaB promoter activity in vivo
      Title: Downregulation of the Escherichia coli guaB promoter by FIS.
    3. The CBS subdomain of IMPDH may coordinate the activities of the enzymes of purine nucleotide metabolism and is essential for maintaining the normal ATP and GTP pool sizes in E. coli.
      Title: The CBS subdomain of inosine 5'-monophosphate dehydrogenase regulates purine nucleotide turnover.
    4. N-terminus verified by Edman degradation on complete protein
      Title: '98 Escherichia coli SWISS-2DPAGE database update.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables C-rich single-stranded DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables IMP dehydrogenase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables IMP dehydrogenase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables IMP dehydrogenase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables guanosine tetraphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables single-stranded DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in GMP biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within GMP biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in GTP biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within protein homotetramerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in purine nucleotide biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to UV IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol HDA PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    inosine 5'-monophosphate dehydrogenase
    NP_417003.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2632604..2634070 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417003.1 inosine 5'-monophosphate dehydrogenase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417003.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0AAN3TRY1
      Conserved Domains (1) summary
      PRK05567
      Location:1 → 487
      PRK05567; inosine 5'-monophosphate dehydrogenase; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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