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    recD exodeoxyribonuclease V subunit RecD [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947287, updated on 3-Dec-2024

    Summary

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    Official Symbol
    recD
    Official Full Name
    exodeoxyribonuclease V subunit RecD
    Primary source
    ECOCYC:EG10826
    Locus tag
    b2819
    See related
    ASAP:ABE-0009247
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2815; hopE
    Summary
    RecBCD is a bipolar helicase. [More information is available at EcoGene: EG10826]. RecB, RecC, and RecD make up a helicase/nuclease complex that is essential for both homologous recombination during the course of transduction and conjugation as well as in repair of double-strand breaks in Escherichia coli . [More information is available at EcoCyc: EG10826].
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    Genomic context

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    Sequence:
    NC_000913.3 (2950635..2952461, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene N-acetylmuramoyl-L-alanine amidase C Neighboring gene N-acetylglutamate synthase Neighboring gene exodeoxyribonuclease V subunit RecB Neighboring gene protease 3 Neighboring gene exodeoxyribonuclease V subunit RecC

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. RecD plays an essential function during growth at low temperature in the antarctic bacterium Pseudomonas syringae Lz4W. Regha K, et al. Genetics, 2005 Aug. PMID 15956672, Free PMC Article
    2. Evidence that recBC-dependent degradation of duplex DNA in Escherichia coli recD mutants involves DNA unwinding. Rinken R, et al. J Bacteriol, 1992 Aug. PMID 1322885, Free PMC Article
    3. RecBCD coordinates repair of two ends at a DNA double-strand break, preventing aberrant chromosome amplification. White MA, et al. Nucleic Acids Res, 2018 Jul 27. PMID 29901759, Free PMC Article
    4. Linear multimer formation of plasmid DNA in Escherichia coli hopE (recD) mutants. Niki H, et al. Mol Gen Genet, 1990 Oct. PMID 2177520
    5. The RecD subunit of the Escherichia coli RecBCD enzyme inhibits RecA loading, homologous recombination, and DNA repair. Amundsen SK, et al. Proc Natl Acad Sci U S A, 2000 Jun 20. PMID 10840065, Free PMC Article

    See all (118) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Chi hotspot control of RecBCD helicase-nuclease by long-range intramolecular signaling.
      Title: Chi hotspot control of RecBCD helicase-nuclease by long-range intramolecular signaling.
    2. Data show that RecBCD and its subunits are able to generate forces up to 25-40 pN without a significant effect on their velocity.
      Title: Synergy between RecBCD subunits is essential for efficient DNA unwinding.
    3. A recD null mutant is defective for the repair of a two-ended DNA double-strand break and that this defect is associated with unregulated chromosome amplification and defective chromosome segregation.
      Title: RecBCD coordinates repair of two ends at a DNA double-strand break, preventing aberrant chromosome amplification.
    4. Data show genetic analysis RecBCD and naive adaptation in Escherichia coli.
      Title: CRISPR-Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5' ssDNA exonucleases.
    5. Extension of the 5'-tail of the unwound duplex induces a large conformational change in the RecD subunit, that is transferred through the RecC subunit to activate the nuclease domain of the RecB subunit.
      Title: Mechanism for nuclease regulation in RecBCD.
    6. A new role of the RecBCD complex in the processing of DNA single-strand gaps that are generated at DNA replication-blocking lesions: independently of its nuclease or helicase activities, the entire RecBCD complex is required for recombinational repair of the gap and efficient translesion synthesis.
      Title: A non-catalytic role of RecBCD in homology directed gap repair and translesion synthesis.
    7. RecBCD is required to complete chromosomal replication. (Review)
      Title: RecBCD is required to complete chromosomal replication: Implications for double-strand break frequencies and repair mechanisms.
    8. Studies characterized the chromosomal replication and degradation patterns during thymine starvation in Rec+ cells, as well as in the recA and recBCD mutants.
      Title: Disintegration of nascent replication bubbles during thymine starvation triggers RecA- and RecBCD-dependent replication origin destruction.
    9. Study hypothesized that RuvAB catalyzes replication fork reversal, RecJ and XonA blunt the double-strand end, and then RecBCD loads RecA4142 onto this end to produce SOS(Con) expression.
      Title: RecA4142 causes SOS constitutive expression by loading onto reversed replication forks in Escherichia coli K-12.
    10. Findings suggest that the recB-recD fusion may form a RecBD fusion protein and therefore affect RecD assembly.
      Title: Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coli.
    Submit: New GeneRIF Correction See all GeneRIFs (22)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 5'-3' DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H2AZ histone chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H3-H4 histone complex chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA clamp loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables chromatin extrusion motor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cohesin loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables exodeoxyribonuclease V activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables exodeoxyribonuclease V activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables exonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables single-stranded DNA helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables single-stranded DNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA damage response IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA duplex unwinding IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA recombination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within DNA recombination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin looping IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin remodeling IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in double-strand break repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in double-strand break repair via homologous recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within double-strand break repair via homologous recombination TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in recombinational repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of exodeoxyribonuclease V complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of exodeoxyribonuclease V complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of exodeoxyribonuclease V complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of exodeoxyribonuclease V complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    exodeoxyribonuclease V subunit RecD
    NP_417296.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2950635..2952461 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417296.1 exodeoxyribonuclease V subunit RecD [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417296.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6N0IMZ1
      Conserved Domains (1) summary
      PRK10875
      Location:3 → 608
      recD; exodeoxyribonuclease V subunit alpha
    Nucleotide Protein
    Heading Accession and Version

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