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    dkgA methylglyoxal reductase DkgA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947495, updated on 3-Dec-2024

    Summary

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    Official Symbol
    dkgA
    Official Full Name
    methylglyoxal reductase DkgA
    Primary source
    ECOCYC:G7565
    Locus tag
    b3012
    See related
    ASAP:ABE-0009892
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3004; yqhE
    Summary
    DkgA has been shown to have a stereoselective beta-keto ester reductase and methylglyxoal reductase activity. [More information is available at EcoCyc: G7565].
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    Genomic context

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    Sequence:
    NC_000913.3 (3156623..3157450)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional activator YqhC Neighboring gene NADPH-dependent aldehyde reductase YqhD Neighboring gene DUF3828 domain-containing protein YqhG Neighboring gene lipoprotein YqhH

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Crystal structure of Escherichia coli DkgA, a broad-specificity aldo-keto reductase. Jeudy S, et al. Proteins, 2006 Jan 1. PMID 16284956
    2. Purification and identification of an Escherichia coli beta-keto ester reductase as 2,5-diketo-D-gluconate reductase YqhE. Habrych M, et al. Biotechnol Prog, 2002 Mar-Apr. PMID 11934293
    3. Silencing of NADPH-dependent oxidoreductase genes (yqhD and dkgA) in furfural-resistant ethanologenic Escherichia coli. Miller EN, et al. Appl Environ Microbiol, 2009 Jul. PMID 19429550, Free PMC Article
    4. Identification of the yqhE and yafB genes encoding two 2, 5-diketo-D-gluconate reductases in Escherichia coli. Yum DY, et al. Appl Environ Microbiol, 1999 Aug. PMID 10427017, Free PMC Article
    5. Conversion of methylglyoxal to acetol by Escherichia coli aldo-keto reductases. Ko J, et al. J Bacteriol, 2005 Aug. PMID 16077126, Free PMC Article

    See all (12) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Purified enzymes encoded by yqhD and dkgA were shown to have NADPH-dependent furfural reductase activity.
      Title: Silencing of NADPH-dependent oxidoreductase genes (yqhD and dkgA) in furfural-resistant ethanologenic Escherichia coli.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: Purification and identification of an Escherichia coli beta-keto ester reductase as 2,5-diketo-D-gluconate reductase YqhE.
    3. Crystallization and xray diffraction of the recombinant protein.
      Title: Crystal structure of Escherichia coli DkgA, a broad-specificity aldo-keto reductase.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 2,5-didehydrogluconate reductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables alcohol dehydrogenase (NADP+) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables aldose reductase (NADPH) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in L-ascorbic acid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in methylglyoxal catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within methylglyoxal catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in small molecule metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    methylglyoxal reductase DkgA
    NP_417485.4

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3156623..3157450
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417485.4 methylglyoxal reductase DkgA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417485.4

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9ANZ1
      Conserved Domains (1) summary
      PRK11565
      Location:1 → 275
      dkgA; 2,5-didehydrogluconate reductase DkgA
    Nucleotide Protein
    Heading Accession and Version

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