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    rpsL 30S ribosomal subunit protein S12 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947845, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rpsL
    Official Full Name
    30S ribosomal subunit protein S12
    Primary source
    ECOCYC:EG10911
    Locus tag
    b3342
    See related
    ASAP:ABE-0010919
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    asuB; ECK3329; strA
    Summary
    Mutations in regions 41-45 (except when lys42 is replaced with arg), 87-93 and 53 result in streptomycin resistance. [More information is available at EcoGene: EG10911]. The S12 protein is a component of the 30S subunit of the ribosome and plays a role in translational accuracy. [More information is available at EcoCyc: EG10911].
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    Genomic context

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    Sequence:
    NC_000913.3 (3474178..3474552, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene elongation factor G Neighboring gene 30S ribosomal subunit protein S7 Neighboring gene sulfurtransferase complex subunit TusB Neighboring gene sulfurtransferase complex subunit TusC Neighboring gene sulfurtransferase complex subunit TusD

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A mutation in the ribosomal protein uS12 reveals novel functions of its universally conserved PNSA loop. Datta M, et al. Mol Microbiol, 2021 Jun. PMID 33368752
    2. A ribosomal function is necessary for efficient splicing of the T4 phage thymidylate synthase intron in vivo. Semrad K, et al. Genes Dev, 1998 May 1. PMID 9573049, Free PMC Article
    3. Strand asymmetry of +1 frameshift mutagenesis at a homopolymeric run by DNA polymerase III holoenzyme of Escherichia coli. Seki M, et al. J Biol Chem, 1999 Nov 19. PMID 10559208
    4. Codon-specific missense errors in vivo. Bouadloun F, et al. EMBO J, 1983. PMID 10872330, Free PMC Article
    5. Escherichia coli proteins, including ribosomal protein S12, facilitate in vitro splicing of phage T4 introns by acting as RNA chaperones. Coetzee T, et al. Genes Dev, 1994 Jul 1. PMID 7958841

    See all (90) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A mutation in the ribosomal protein uS12 reveals novel functions of its universally conserved PNSA loop.
      Title: A mutation in the ribosomal protein uS12 reveals novel functions of its universally conserved PNSA loop.
    2. The semi error-restrictive mutation (K87R), within the rpsL gene, causes an increase in the abundance and activity of proteins encoded by exogenous genes containing non-optimal AT-rich codons in E. coli.
      Title: Ribosomal mutations affecting the translation of genes that use non-optimal codons.
    3. The authors provide evidence that mutant IF2 specifically interacts with rpsL31 in cold, leading to a bacteriostatic effect on host cells.
      Title: A novel mutation spatially remote from the G-domain in IF2 affects the cold stress adaptation of Escherichia coli.
    4. K42A and R53A variants of RpsL, were substantially resistant to the miscoding effects of paromomycin.
      Title: Mutational analysis of S12 protein and implications for the accuracy of decoding by the ribosome.
    5. N-terminus verified by Edman degradation on mature peptide
      Title: Primary stucture of protein S12 from the small Escherichia coli ribosomal subunit.
    6. combination of S12 & otherwise benign mutations at positions C1409-G1491 in 16S rRNA severely compromises cell growth while level & range of aminoglycoside resistances conferred by the G1491U/C substitutions is markedly increased by a mutant S12 protein.
      Title: Modulation of 16S rRNA function by ribosomal protein S12.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables misfolded RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables rRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables rRNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables structural constituent of ribosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables structural constituent of ribosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables structural constituent of ribosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within Group I intron splicing IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within RNA folding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in cytoplasmic translation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in maintenance of translational fidelity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of RNA splicing IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in response to antibiotic IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of cytosolic small ribosomal subunit IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of cytosolic small ribosomal subunit IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of ribonucleoprotein complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in ribosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in ribosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of small ribosomal subunit IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    30S ribosomal subunit protein S12

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3474178..3474552 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417801.1 30S ribosomal subunit protein S12 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417801.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6C9QFZ1
      Conserved Domains (1) summary
      PRK05163
      Location:1 → 124
      rpsL; 30S ribosomal protein S12; Validated
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

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    Other Literature Sources
    Molecular Biology Databases
    Research Materials