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    trmA tRNA m(5)U54 methyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947143, updated on 3-Dec-2024

    Summary

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    Official Symbol
    trmA
    Official Full Name
    tRNA m(5)U54 methyltransferase
    Primary source
    ECOCYC:EG11022
    Locus tag
    b3965
    See related
    ASAP:ABE-0012983
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3957; rT; rumT
    Summary
    The trmA gene is essential. [More information is available at EcoGene: EG11022]. trmA encodes the methyltransferase that is responsible for methylation of U54 in all tRNAs. [More information is available at EcoCyc: EG11022].
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    Genomic context

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    Sequence:
    NC_000913.3 (4162170..4163270, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional dual regulator OxyR Neighboring gene soluble pyridine nucleotide transhydrogenase Neighboring gene DNA-binding transcriptional repressor FabR Neighboring gene DUF1422 domain-containing inner membrane protein YijD Neighboring gene cobalamin outer membrane transporter Neighboring gene glutamate racemase Neighboring gene 16S ribosomal RNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Purification of transfer RNA (m5U54)-methyltransferase from Escherichia coli. Association with RNA. Ny T, et al. Eur J Biochem, 1988 Nov 15. PMID 2461858
    2. Enzymatic mechanism of tRNA (m5U54)methyltransferase. Kealey JT, et al. Biochimie, 1994. PMID 7748948
    3. Stereochemistry of tRNA(m5U54)-methyltransferase catalysis: 19F NMR spectroscopy of an enzyme-FUraRNA covalent complex. Kealey JT, et al. Biochemistry, 1995 Feb 28. PMID 7532997
    4. The methyltransferase TrmA facilitates tRNA folding through interaction with its RNA-binding domain. Keffer-Wilkes LC, et al. Nucleic Acids Res, 2020 Aug 20. PMID 32597953, Free PMC Article
    5. Cloning and restriction mapping of the trmA gene coding for transfer ribonucleic acid (5-methyluridine)-methyltransferase in Escherichia coli K-12. Ny T, et al. J Bacteriol, 1980 May. PMID 6247318, Free PMC Article

    See all (50) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The methyltransferase TrmA facilitates tRNA folding through interaction with its RNA-binding domain.
      Title: The methyltransferase TrmA facilitates tRNA folding through interaction with its RNA-binding domain.
    2. findings show that m (5)U formation in tmRNAs is exclusively due to TrmA and should be considered as a dual-specific enzyme
      Title: RNA-methyltransferase TrmA is a dual-specific enzyme responsible for C5-methylation of uridine in both tmRNA and tRNA.
    3. N-terminus verified by Edman degradation on complete protein
      Title: Purification of transfer RNA (m5U54)-methyltransferase from Escherichia coli. Association with RNA.
    4. A mutagenesis study to determine which amino acids are important for the formation of the TrmA-tRNA intermediate and/or the enzymatic activity.
      Title: Amino acid residues of the Escherichia coli tRNA(m5U54)methyltransferase (TrmA) critical for stability, covalent binding of tRNA and enzymatic activity.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables RNA methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables S-adenosylmethionine-dependent methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables rRNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables rRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables tRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in RNA modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in RNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tRNA folding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tRNA methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tRNA methylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    tRNA m(5)U54 methyltransferase
    NP_418400.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4162170..4163270 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418400.1 tRNA m(5)U54 methyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418400.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376L3L6, W1F562
      Conserved Domains (1) summary
      PRK05031
      Location:1 → 366
      PRK05031; tRNA (uracil-5-)-methyltransferase; Validated
    Nucleotide Protein
    Heading Accession and Version

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