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    thiF sulfur carrier protein ThiS adenylyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948500, updated on 17-Dec-2024

    Summary

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    Official Symbol
    thiF
    Official Full Name
    sulfur carrier protein ThiS adenylyltransferase
    Primary source
    ECOCYC:EG11587
    Locus tag
    b3992
    See related
    ASAP:ABE-0013053
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3984; thiA
    Summary
    The ThiF sequence has been confirmed using MS/MS, however since use of the start codon was forced during cloning, this is not a verification of the start site: the measured mass of the intact protein is 26970.2 indicating that the initial Met is not removed (Kelleher, 1998). [More information is available at EcoGene: EG11587]. The ThiF protein catalyzes the adenylation of the ThiS protein as part of the formation of the thiazole moiety during thiamin biosynthesis . [More information is available at EcoCyc: EG11587].
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    Genomic context

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    Sequence:
    NC_000913.3 (4192821..4193576, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene 2-iminoacetate synthase Neighboring gene 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase Neighboring gene sulfur carrier protein ThiS Neighboring gene thiamine phosphate synthase Neighboring gene phosphomethylpyrimidine synthase Neighboring gene regulator of sigma D

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structural analysis of Escherichia coli ThiF. Duda DM, et al. J Mol Biol, 2005 Jun 17. PMID 15896804
    2. Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: identification of an acyldisulfide-linked protein--protein conjugate that is functionally analogous to the ubiquitin/E1 complex. Xi J, et al. Proc Natl Acad Sci U S A, 2001 Jul 17. PMID 11438688, Free PMC Article
    3. Structure of the Escherichia coli ThiS-ThiF complex, a key component of the sulfur transfer system in thiamin biosynthesis. Lehmann C, et al. Biochemistry, 2006 Jan 10. PMID 16388576, Free PMC Article
    4. Thiamin biosynthesis in Escherichia coli. Identification of ThiS thiocarboxylate as the immediate sulfur donor in the thiazole formation. Taylor SV, et al. J Biol Chem, 1998 Jun 26. PMID 9632726
    5. The enzymology of sulfur activation during thiamin and biotin biosynthesis. Begley TP, et al. Curr Opin Chem Biol, 1999 Oct. PMID 10508664

    See all (17) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. crystal structures of ThiF, alone and in complex with ATP
      Title: Structural analysis of Escherichia coli ThiF.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables AMPylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotidyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables nucleotidyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sulfotransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables thiosulfate sulfurtransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin-like modifier activating enzyme activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in thiamine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in thiamine diphosphate biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within thiazole biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of adenylyltransferase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of sulfurtransferase complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    sulfur carrier protein ThiS adenylyltransferase
    NP_418420.4

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4192821..4193576 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418420.4 sulfur carrier protein ThiS adenylyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418420.4

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1V2G5P4, A0A8S7CSR0
      Conserved Domains (1) summary
      TIGR02356
      Location:8 → 209
      adenyl_thiF; thiazole biosynthesis adenylyltransferase ThiF, E. coli subfamily
    Nucleotide Protein
    Heading Accession and Version

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