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    xylE D-xylose:H(+) symporter [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948529, updated on 3-Dec-2024

    Summary

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    Official Symbol
    xylE
    Official Full Name
    D-xylose:H(+) symporter
    Primary source
    ECOCYC:EG11076
    Locus tag
    b4031
    See related
    ASAP:ABE-0013189
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4023
    Summary
    Major Facilitator Superfamily (MFS). [More information is available at EcoGene: EG11076]. XylE is a D-xylose/proton symporter, one of two systems in Escherichia coli responsible for the uptake of D-xylose. [More information is available at EcoCyc: EG11076].
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    Genomic context

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    Sequence:
    NC_000913.3 (4240779..4242254, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene PF17089 family protein YjbT Neighboring gene putative phosphate starvation-inducible protein PsiE Neighboring gene ncRNA Neighboring gene maltose ABC transporter membrane subunit MalG Neighboring gene maltose ABC transporter membrane subunit MalF

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Crystal structure of a bacterial homologue of glucose transporters GLUT1-4. Sun L, et al. Nature, 2012 Oct 18. PMID 23075985
    2. Proton-linked D-xylose transport in Escherichia coli. Lam VM, et al. J Bacteriol, 1980 Jul. PMID 6995439, Free PMC Article
    3. Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter. Jia R, et al. Nat Commun, 2020 Dec 2. PMID 33268777, Free PMC Article
    4. Structural basis for substrate transport in the GLUT-homology family of monosaccharide transporters. Quistgaard EM, et al. Nat Struct Mol Biol, 2013 Jun. PMID 23624861
    5. Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE. Wisedchaisri G, et al. Nat Commun, 2014 Aug 4. PMID 25088546, Free PMC Article

    See all (32) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter.
      Title: Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter.
    2. Molecular dynamics (MD) study of an all-atom model system to elucidate the atomistic details and the free-energy landscape along the path of binding a xylopyranose from the extracellular space to the inside of the transporter protein XylE. From the MD simulations, the Gibbs free energy of binding was found to be -4.4kcal/mol in agreement with the experimental value of -4.7kcal/mol.
      Title: Affinity and path of binding xylopyranose unto E. coli xylose permease.
    3. The models and subsequent experimental validation suggest that multiple residue substitutions are required to produce the thermodynamic and functional distinction between XylE and GLUT1. Despite the lack of simulation studies with substrates, these computational and biochemical characterizations provide unprecedented insight into the mechanistic difference between proton symporters and uniporters.
      Title: Molecular determinants for the thermodynamic and functional divergence of uniporter GLUT1 and proton symporter XylE.
    4. studies suggest that a single carboxyl group in LacY (Glu325) may be the only side chain directly involved in H+ translocation and a carboxyl side chain with similar properties has been identified in FucP (Asp46) and XylE (Asp27), the present results imply that the pK of this residue is switched during H+/sugar symport in all three symporters.
      Title: pH Regulation of Electrogenic Sugar/H+ Symport in MFS Sugar Permeases.
    5. Studied conformational states and homology of LacY and XylE.
      Title: Conserved movement of TMS11 between occluded conformations of LacY and XylE of the major facilitator superfamily suggests a similar hinge-like mechanism.
    6. The movement of XylE is described that facilitates sugar translocation across a lipid membrane and identify the likely candidate proton-coupling residues as the conserved Asp27 and Arg133.
      Title: Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE.
    7. This current model helps to unify the apparently opposing concepts of alternate access and multisite models of ligand transport.
      Title: Reptation-induced coalescence of tunnels and cavities in Escherichia Coli XylE transporter conformers accounts for facilitated diffusion.
    8. Authors present two structures of the major facilitator (MFS) xylose transporter XylE from Escherichia coli in inward open and partially occluded inward open conformations.
      Title: Structural basis for substrate transport in the GLUT-homology family of monosaccharide transporters.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables D-xylose:proton symporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables symporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transmembrane transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within D-xylose transmembrane transport EXP
    Inferred from Experiment
    more info
    		 PubMed 
    acts_upstream_of_or_within D-xylose transmembrane transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    D-xylose:H(+) symporter

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4240779..4242254 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418455.1 D-xylose:H(+) symporter [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418455.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A831GRH0
      Conserved Domains (1) summary
      PRK10077
      Location:1 → 485
      xylE; D-xylose transporter XylE; Provisional
    Nucleotide Protein
    Heading Accession and Version

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