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    nrfA cytochrome c552 nitrite reductase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948571, updated on 17-Dec-2024

    Summary

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    Official Symbol
    nrfA
    Official Full Name
    cytochrome c552 nitrite reductase
    Primary source
    ECOCYC:EG11781
    Locus tag
    b4070
    See related
    ASAP:ABE-0013342
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4063
    Summary
    The gene nrfA is the structural gene for cytochrome C552, which functions as a formate-dependent nitrite reductase. [More information is available at EcoCyc: EG11781].
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    Genomic context

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    Sequence:
    NC_000913.3 (4287764..4289200)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene acetyl-CoA synthetase (AMP-forming) Neighboring gene protein YtcB Neighboring gene periplasmic nitrite reductase penta-heme c-type cytochrome Neighboring gene putative menaquinol-cytochrome c reductase 4Fe-4S subunit Neighboring gene putative menaquinol-cytochrome c reductase subunit NrfD

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Kinetic and thermodynamic resolution of the interactions between sulfite and the pentahaem cytochrome NrfA from Escherichia coli. Kemp GL, et al. Biochem J, 2010 Oct 1. PMID 20629638
    2. Purification of a hexaheme cytochrome c552 from Escherichia coli K 12 and its properties as a nitrite reductase. Kajie S, et al. Eur J Biochem, 1986 Jan 15. PMID 3002798
    3. Spectroelectrochemical characterization of a pentaheme cytochrome in solution and as electrocatalytically active films on nanocrystalline metal-oxide electrodes. Marritt SJ, et al. J Am Chem Soc, 2008 Jul 9. PMID 18549208
    4. Escherichia coli cytochrome c nitrite reductase NrfA. Clarke TA, et al. Methods Enzymol, 2008. PMID 18433623
    5. Studies on soluble cytochromes in Enterobacteriaceae. I. Detection, purification, and properties of cytochrome c-552 in anaerobically grown cells. Fujita T. J Biochem, 1966 Aug. PMID 5338848

    See all (40) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Elucidating Electron Storage and Distribution within the Pentaheme Scaffold of Cytochrome c Nitrite Reductase (NrfA).
      Title: Elucidating Electron Storage and Distribution within the Pentaheme Scaffold of Cytochrome c Nitrite Reductase (NrfA).
    2. Kinetic and thermodynamic resolution of the interactions between sulfite and the pentahaem cytochrome NrfA from Escherichia coli
      Title: Kinetic and thermodynamic resolution of the interactions between sulfite and the pentahaem cytochrome NrfA from Escherichia coli.
    3. Activation of the Escherichia coli K-12 nrf promoter is downregulated by the nitric oxide-sensitive NsrR protein together with the nucleoid-associated protein IHF.
      Title: Down-regulation of the Escherichia coli K-12 nrf promoter by binding of the NsrR nitric oxide-sensing transcription repressor to an upstream site.
    4. Spectroelectrochemical studies of E. coli NrfA have provided unprecedented resolution of its thermodynamic properties.
      Title: Spectroelectrochemical characterization of a pentaheme cytochrome in solution and as electrocatalytically active films on nanocrystalline metal-oxide electrodes.
    5. Method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.
      Title: Escherichia coli cytochrome c nitrite reductase NrfA.
    6. N-terminus verified by Edman degradation on mature peptide
      Title: Regulation and sequence of the structural gene for cytochrome c552 from Escherichia coli: not a hexahaem but a 50 kDa tetrahaem nitrite reductase.
    7. Protein-protein interaction studies suggest NrfA and NrfB can form a 20-haem NrfA2-NrfB2 heterotetrameric complex.
      Title: The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables calcium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables heme binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables heme binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables heme binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables iron ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nitric oxide reductase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nitrite reductase (cytochrome, ammonia-forming) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables nitrite reductase (cytochrome, ammonia-forming) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nitrite reductase (cytochrome, ammonia-forming) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in anaerobic electron transport chain IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within anaerobic electron transport chain IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in anaerobic respiration IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in nitrate assimilation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in outer membrane-bounded periplasmic space IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in outer membrane-bounded periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in periplasmic space IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    cytochrome c552 nitrite reductase

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4287764..4289200
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418494.1 cytochrome c552 nitrite reductase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418494.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A403CWJ2, A0AAP3EHY4
      Conserved Domains (1) summary
      PRK11125
      Location:4 → 478
      nrfA; ammonia-forming cytochrome c nitrite reductase
    Nucleotide Protein
    Heading Accession and Version

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