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    ahpF alkyl hydroperoxide reductase, AhpF component [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947540, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ahpF
    Official Full Name
    alkyl hydroperoxide reductase, AhpF component
    Primary source
    ECOCYC:EG11385
    Locus tag
    b0606
    See related
    ASAP:ABE-0002093
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0600
    Summary
    The native AhpF from Salmonella has been N-terminally sequenced (Jacobson et al, 1989), supporting the choice of this alternate initiation codon in Escherichia coli by inference. [More information is available at EcoGene: EG11385]. The AhpF component channels electrons from NAD(P)H via a series of disulfides to the AhpC component for reduction of the hydroperoxide substrate. [More information is available at EcoCyc: EG11385].
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    Genomic context

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    Sequence:
    NC_000913.3 (639753..641318)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein sulfenic acid reductase and chaperone DsbG Neighboring gene alkyl hydroperoxide reductase, AhpC component Neighboring gene universal stress protein G Neighboring gene putative Zn(2(+))-dependent alcohol dehydrogenase YbdR

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Crystal structure of the catalytic core component of the alkylhydroperoxide reductase AhpF from Escherichia coli. Bieger B, et al. J Mol Biol, 2001 Mar 16. PMID 11243797
    2. Structure of intact AhpF reveals a mirrored thioredoxin-like active site and implies large domain rotations during catalysis. Wood ZA, et al. Biochemistry, 2001 Apr 3. PMID 11300769
    3. Structure, mechanism and ensemble formation of the alkylhydroperoxide reductase subunits AhpC and AhpF from Escherichia coli. Dip PV, et al. Acta Crystallogr D Biol Crystallogr, 2014 Nov. PMID 25372677
    4. Locations of genes encoding alkyl hydroperoxide reductase on the physical map of the Escherichia coli K-12 genome. Smillie DA, et al. J Bacteriol, 1992 Jun. PMID 1592833, Free PMC Article
    5. An alkyl hydroperoxide reductase from Salmonella typhimurium involved in the defense of DNA against oxidative damage. Purification and properties. Jacobson FS, et al. J Biol Chem, 1989 Jan 25. PMID 2643600

    See all (36) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. a concerted movement involving the NTD, C-terminal NADH, and FAD domains, and the flexible linker between them in AhpF is essential for optimal intra-domain cross-talk and for efficient electron transfer to the redox partner AhpC required for peroxidation
      Title: Essential role of the flexible linker on the conformational equilibrium of bacterial peroxiredoxin reductase for effective regeneration of peroxiredoxin.
    2. Oxidative stress response proteins such as AhpF, Dps, and Fur, all of which belong to the oxyR regulon, were upregulated in Escherichia coli isolates from mice fed the lactose-rich diet.
      Title: Impact of nutritional factors on the proteome of intestinal Escherichia coli: induction of OxyR-dependent proteins AhpF and Dps by a lactose-rich diet.
    3. Hpx- (katG katE ahpCF) mutants of Escherichia coli have DNA damage from submicromolar intracellular hydrogen peroxide
      Title: Substantial DNA damage from submicromolar intracellular hydrogen peroxide detected in Hpx- mutants of Escherichia coli.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables FAD binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables NAD binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables NADH-dependent peroxiredoxin activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables alkyl hydroperoxide reductase activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    enables flavin adenine dinucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables thioredoxin-disulfide reductase (NADPH) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in cell redox homeostasis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in cellular oxidant detoxification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in hydrogen peroxide catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in response to oxidative stress NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in response to reactive oxygen species IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of alkyl hydroperoxide reductase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of alkyl hydroperoxide reductase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytosol HDA PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    alkyl hydroperoxide reductase, AhpF component
    NP_415139.2

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      639753..641318
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415139.2 alkyl hydroperoxide reductase, AhpF component [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415139.2

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A776EKQ4, A0A8S7X5E7
      Conserved Domains (1) summary
      PRK15317
      Location:1 → 521
      PRK15317; alkyl hydroperoxide reductase subunit F; Provisional
    Nucleotide Protein
    Heading Accession and Version

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