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    norV anaerobic nitric oxide reductase flavorubredoxin [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948979, updated on 17-Dec-2024

    Summary

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    Official Symbol
    norV
    Official Full Name
    anaerobic nitric oxide reductase flavorubredoxin
    Primary source
    ECOCYC:G7413
    Locus tag
    b2710
    See related
    ASAP:ABE-0008910
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2705; ygaI; ygaJ; ygaK
    Summary
    Upregulated by GSNO in defined medium. [More information is available at EcoGene: EG12963]. Flavorubredoxin (FlRd) is a multidomain protein containing an amino-terminal -lactamase-like module with a non-heme di-iron site as the catalytic center, a short chain flavodoxin-like module and a rubredoxin-like extension. [More information is available at EcoCyc: G7413].
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    Genomic context

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    Sequence:
    NC_000913.3 (2832476..2833915)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional repressor SrlR Neighboring gene D-arabinose 5-phosphate isomerase GutQ Neighboring gene DNA-binding transcriptional dual regulator NorR Neighboring gene NADH:flavorubredoxin reductase Neighboring gene carbamoyl--[HypE] ligase Neighboring gene putative electron transport protein HydN

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Oxidative stress modulates the nitric oxide defense promoted by Escherichia coli flavorubredoxin. Baptista JM, et al. J Bacteriol, 2012 Jul. PMID 22563051, Free PMC Article
    2. Quaternary structure of flavorubredoxin as revealed by synchrotron radiation small-angle X-ray scattering. Petoukhov MV, et al. Structure, 2008 Sep 10. PMID 18786405
    3. Kinetic characterization of the Escherichia coli nitric oxide reductase flavorubredoxin. Vicente JB, et al. Methods Enzymol, 2008. PMID 18433622
    4. Kinetics of electron transfer from NADH to the Escherichia coli nitric oxide reductase flavorubredoxin. Vicente JB, et al. FEBS J, 2007 Feb. PMID 17181540
    5. Structure of Escherichia coli Flavodiiron Nitric Oxide Reductase. Romão CV, et al. J Mol Biol, 2016 Nov 20. PMID 27725182

    See all (24) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The overall tetrameric structure revealed a highly conserved flavodiiron core domain, with a metallo-beta-lactamase-like domain containing a diiron center, and a flavodoxin domain with a flavin mononucleotide cofactor.
      Title: Structure of Escherichia coli Flavodiiron Nitric Oxide Reductase.
    2. Rubredoxin binds nine TPR motifs to form LapB, an essential regulator of lipopolysaccharide synthesis.
      Title: An Unexpected Duo: Rubredoxin Binds Nine TPR Motifs to Form LapB, an Essential Regulator of Lipopolysaccharide Synthesis.
    3. Authors propose that the time-dependent activation of flavorubredoxin contributes to the adaptation of E. coli to the different fluxes of hydrogen peroxide and nitric oxide to which the bacterium is subjected during the course of macrophage infection.
      Title: Oxidative stress modulates the nitric oxide defense promoted by Escherichia coli flavorubredoxin.
    4. Quaternary structure of flavorubredoxin as revealed by synchrotron radiation small-angle X-ray scattering.
      Title: Quaternary structure of flavorubredoxin as revealed by synchrotron radiation small-angle X-ray scattering.
    5. the atypical glutamate does not guide metal selectivity of the FlRd metallo-beta-lactamase domain but suppresses possible hydrolytic cross-activity
      Title: Zinc- and iron-dependent cytosolic metallo-beta-lactamase domain proteins exhibit similar zinc-binding affinities, independent of an atypical glutamate at the metal-binding site.
    6. biochemical analyis of FlRd and its physiological partner, the NADH:flavorubredoxin oxidoreductase FlRd-Red
      Title: Redox and spectroscopic properties of the Escherichia coli nitric oxide-detoxifying system involving flavorubredoxin and its NADH-oxidizing redox partner.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables FMN binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables FMN binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables electron transfer activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables electron transfer activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables iron ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables iron ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nitric oxide reductase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nitric oxide reductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity, acting on other nitrogenous compounds as donors IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within nitric oxide catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to nitric oxide IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    anaerobic nitric oxide reductase flavorubredoxin

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2832476..2833915
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417190.1 anaerobic nitric oxide reductase flavorubredoxin [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417190.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376M547, A0A797LYQ3
      Conserved Domains (1) summary
      PRK05452
      Location:1 → 479
      PRK05452; anaerobic nitric oxide reductase flavorubredoxin; Provisional
    Nucleotide Protein
    Heading Accession and Version

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