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    metC cystathionine beta-lyase/L-cysteine desulfhydrase/alanine racemase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946240, updated on 3-Dec-2024

    Summary

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    Official Symbol
    metC
    Official Full Name
    cystathionine beta-lyase/L-cysteine desulfhydrase/alanine racemase
    Primary source
    ECOCYC:EG10583
    Locus tag
    b3008
    See related
    ASAP:ABE-0009878
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3000
    Summary
    The Flint et al. [More information is available at EcoGene: EG10583]. Several distinct proteins exhibit cysteine desulfhydrase activity, which is involved in cysteine degradation . [More information is available at EcoCyc: EG10583].
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    Genomic context

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    Sequence:
    NC_000913.3 (3152236..3153423)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene Ton complex subunit ExbD Neighboring gene Ton complex subunit ExbB Neighboring gene DedA family protein YghB Neighboring gene DNA-binding transcriptional activator YqhC

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Cystathionine β-lyase is involved in d-amino acid metabolism. Miyamoto T, et al. Biochem J, 2018 Apr 23. PMID 29592871
    2. Characterization of the side-chain hydroxyl moieties of residues Y56, Y111, Y238, Y338, and S339 as determinants of specificity in E. coli cystathionine β-lyase. Lodha PH, et al. Biochemistry, 2011 Nov 15. PMID 21958132
    3. Genetic characterization of the metK locus in Escherichia coli K-12. Hunter JS, et al. J Bacteriol, 1975 Jun. PMID 1097390, Free PMC Article
    4. Cloning, purification, and characterization of beta-cystathionase from Escherichia coli. Dwivedi CM, et al. Biochemistry, 1982 Jun 22. PMID 7049234
    5. Cloning, purification, and crystallization of Escherichia coli cystathionine beta-lyase. Laber B, et al. FEBS Lett, 1996 Jan 22. PMID 8566238

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. a representative of fold type III, the Escherichia coli alanine racemase (ALR), is a promiscuous cystathionine beta-lyase (CBL). Furthermore, E. coli CBL (fold type I) is a promiscuous alanine racemase. A single round of error-prone PCR and selection yielded variant ALR(Y274F), which catalyzes cystathionine beta-elimination with a near-native Michaelis constant (Km = 3.3 mm) but a poor turnover number (kcat approximate...
      Title: Mechanistic and Evolutionary Insights from the Reciprocal Promiscuity of Two Pyridoxal Phosphate-dependent Enzymes.
    2. A series of site-directed variants of CBL was constructed to investigate the roles of the hydroxyl moieties of active-site residues Y56, Y111, Y238, Y338, and S339 as determinants of specificity.
      Title: Characterization of the side-chain hydroxyl moieties of residues Y56, Y111, Y238, Y338, and S339 as determinants of specificity in E. coli cystathionine β-lyase.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: Studies on the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase in escherichia coli crude extract. Isolation of O-acetylserine sulfhydrylases A and B and beta-cystathionase based on their ability to mobilize sulfur from cysteine and to participate in Fe-S cluster synthesis.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables L-cysteine desulfhydrase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables L-cysteine desulfhydrase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables L-cysteine desulfhydrase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables alanine racemase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables catalytic activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cysteine-S-conjugate beta-lyase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables cysteine-S-conjugate beta-lyase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cysteine-S-conjugate beta-lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables pyridoxal phosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pyridoxal phosphate binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in L-cysteine catabolic process to pyruvate IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within L-cysteine catabolic process to pyruvate IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in methionine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within methionine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within protein homotetramerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in transsulfuration IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    cystathionine beta-lyase/L-cysteine desulfhydrase/alanine racemase
    NP_417481.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3152236..3153423
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417481.1 cystathionine beta-lyase/L-cysteine desulfhydrase/alanine racemase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417481.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2H4TV68
      Conserved Domains (1) summary
      PRK08114
      Location:1 → 395
      PRK08114; cystathionine beta-lyase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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