parE DNA topoisomerase IV subunit B [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: parE
Official Full Name: DNA topoisomerase IV subunit B
Primary source: ECOCYC:EG10687
Locus tag: b3030
See related: ASAP:ABE-0009950
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3021; nfxD
Summary: Topo IV is stimulated by SeqA. [More information is available at EcoGene: EG10687]. The crystal structures of two N-terminal fragments of ParE have been solved . [More information is available at EcoCyc: EG10687].
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Genomic context

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Sequence:: NC_000913.3 (3173504..3175396, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli.
Title: Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli.
a detailed characterization of a known potent inhibitor containing a 9H-pyrimido[4,5-b]indole scaffold against the N-terminal domain of the topoisomerase IV E subunit from Escherichia coli (eParE). Using a series of biophysical and biochemical experiments, it has been demonstrated that this inhibitor forms a tight complex with eParE.
Title: Escherichia coli Topoisomerase IV E Subunit and an Inhibitor Binding Mode Revealed by NMR Spectroscopy.
This interaction is modulated by MatP, a protein involved in the organization of the Ter macrodomain. These results show that Topo IV, XerCD/dif and MatP are part of a network dedicated to the final step of chromosome management during the cell cycle.
Title: Mapping Topoisomerase IV Binding and Activity Sites on the E. coli Genome.
Impairing the TopoIV-MukBEF interaction delays sister ter segregation in cells lacking MatP.
Title: MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation.
The I529L SNP in parE was found in most, but not all, Escherichia coli ST131 isolates with fluoroquinolone and cephalosporin resistance.
Title: Characterization of fluoroquinolone and cephalosporin resistance mechanisms in Enterobacteriaceae isolated in a Dutch teaching hospital reveals the presence of an Escherichia coli ST131 clone with a specific mutation in parE.
QnrA binds to topoisomerase IV and its subunits in a reaction that does not depend on the presence of DNA, quinolone, or ATP
Title: Interaction of the plasmid-encoded quinolone resistance protein QnrA with Escherichia coli topoisomerase IV.
the E.coli topoisomerase IV conformation is altered upon enzyme binding to positively supercoiled DNA
Title: Alteration of Escherichia coli topoisomerase IV conformation upon enzyme binding to positively supercoiled DNA.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA negative supercoiling activity	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase activity	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA topological change	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA topological change	IEA Inferred from Electronic Annotation more info
involved_in chromosome organization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within chromosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within plasmid partitioning	IDA Inferred from Direct Assay more info	PubMed
involved_in response to antibiotic	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex	NAS Non-traceable Author Statement more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA topoisomerase IV subunit B

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3173504..3175396 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417502.1 DNA topoisomerase IV subunit B [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417502.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A376FWG8

Conserved Domains (1) summary

TIGR01055
Location:2 → 626

parE_Gneg; DNA topoisomerase IV, B subunit, proteobacterial