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    clcA chloride:H(+) antiporter ClcA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946715, updated on 3-Dec-2024

    Summary

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    Official Symbol
    clcA
    Official Full Name
    chloride:H(+) antiporter ClcA
    Primary source
    ECOCYC:EG12331
    Locus tag
    b0155
    See related
    ASAP:ABE-0000533
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0154; eriC; yadQ
    Summary
    ClcA and ClcB encode redundant chloride channels. [More information is available at EcoGene: EG12331]. EriC is a homologue of ClC chloride channels. [More information is available at EcoCyc: EG12331].
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    Genomic context

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    Sequence:
    NC_000913.3 (175107..176528)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene glutamate-1-semialdehyde 2,1-aminomutase Neighboring gene protein YadX Neighboring gene protein YadW Neighboring gene iron-sulfur cluster insertion protein ErpA Neighboring gene PF03458 family protein YadS

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Conformational changes required for H(+)/Cl(-) exchange mediated by a CLC transporter. Basilio D, et al. Nat Struct Mol Biol, 2014 May. PMID 24747941, Free PMC Article
    2. Gating the selectivity filter in ClC chloride channels. Dutzler R, et al. Science, 2003 Apr 4. PMID 12649487
    3. Structure and function of clc channels. Chen TY. Annu Rev Physiol, 2005. PMID 15709979
    4. X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity. Dutzler R, et al. Nature, 2002 Jan 17. PMID 11796999
    5. Side-dependent inhibition of a prokaryotic ClC by DIDS. Matulef K, et al. Biophys J, 2005 Sep. PMID 15994902, Free PMC Article

    See all (36) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Thermodynamic analysis of ion and proton binding shows how the structure of the CLC transporter effects the pH-independent, 2 Cl- to 1 proton binding stoichiometry. The key features are a helix cage, which creates a compact region with a very strong electropositive potential that stabilizes two anions and a single Glutamate that competes with two Cl ions.
      Title: Two Cl Ions and a Glu Compete for a Helix Cage in the CLC Proton/Cl(-) Antiporter.
    2. The authors propose that CLC-ec1 operates through an affinity-switch mechanism and that the bases of substrate specificity are conserved in the CLC channels and transporters.
      Title: Basis of substrate binding and conservation of selectivity in the CLC family of channels and transporters.
    3. E203 substitution with Cys and crystal structures of mutants showed that respective Glu is the internal proton-transfer residue that delivers protons from intracellular solution to the protein interior
      Title: Intracellular proton-transfer mutants in a CLC Cl-/H+ exchanger.
    4. Crystallographic study on the ion-binding properties of the ClC selectivity filter in the close homolog from Escherichia coli was presented.
      Title: Ion-binding properties of the ClC chloride selectivity filter.
    5. The unitary Cl(-) transport rate, measured in liposomes by both initial-velocity method and a novel Poisson dilution approach, is approximately 4,000 s(-1) for wild-type protein, and the uncoupled mutants transport Cl(-) at similar rates
      Title: Uncoupling and turnover in a Cl-/H+ exchange transporter.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables antiporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables chloride:proton antiporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables voltage-gated chloride channel activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables voltage-gated chloride channel activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in cellular stress response to acidic pH IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in chloride transmembrane transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in chloride transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in monoatomic ion transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in proton transmembrane transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    chloride:H(+) antiporter ClcA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      175107..176528
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414697.1 chloride:H(+) antiporter ClcA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414697.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6C9Q5G3, A0A8S7TIM9
      Conserved Domains (1) summary
      PRK05277
      Location:36 → 473
      PRK05277; H(+)/Cl(-) exchange transporter ClcA
    Nucleotide Protein
    Heading Accession and Version

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