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    ispA geranyl diphosphate/farnesyl diphosphate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945064, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ispA
    Official Full Name
    geranyl diphosphate/farnesyl diphosphate synthase
    Primary source
    ECOCYC:EG10508
    Locus tag
    b0421
    See related
    ASAP:ABE-0001463
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0415
    Summary
    Farnesyl diphosphate synthase can utilize both dimethylallyl and geranyl diphosphates as substrates, generating geranyl and farnesyl diphosphate, respectively. [More information is available at EcoCyc: EG10508].
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    Genomic context

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    Sequence:
    NC_000913.3 (440202..441101, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 1-deoxyxylulose-5-phosphate synthase YajO Neighboring gene 1-deoxy-D-xylulose-5-phosphate synthase Neighboring gene exodeoxyribonuclease VII subunit XseB Neighboring gene tRNA uridine 4-sulfurtransferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Geranyl diphosphate synthase: an important regulation point in balancing a recombinant monoterpene pathway in Escherichia coli. Zhou J, et al. Enzyme Microb Technol, 2015 Jan. PMID 25435505
    2. A mutation of ispA that is involved in isoprenoid biogenesis can improve growth of Escherichia coli at low temperatures. Shiomi D, et al. Microbiol Immunol, 2011 Dec. PMID 22003871
    3. Short-chain prenyl diphosphate synthase that condenses isopentenyl diphosphate with dimethylallyl diphosphate in ispA null Escherichia coli strain lacking farnesyl diphosphate synthase. Saito K, et al. J Biosci Bioeng, 2007 Jun. PMID 17630132
    4. Isoprenoid biosynthesis as a drug target: bisphosphonate inhibition of Escherichia coli K12 growth and synergistic effects of fosmidomycin. Leon A, et al. J Med Chem, 2006 Dec 14. PMID 17149863
    5. Farnesyl diphosphate synthase: the art of compromise between substrate selectivity and stereoselectivity. Thulasiram HV, et al. J Am Chem Soc, 2006 Dec 13. PMID 17147392, Free PMC Article

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The expression of GPPS was optimized by using ribosomal binding sites designed to have different translation initiation rates. GPPS is an important regulation point in balancing a recombinant geraniol synthesis pathway.
      Title: Geranyl diphosphate synthase: an important regulation point in balancing a recombinant monoterpene pathway in Escherichia coli.
    2. cold-sensitive growth in the rodZ mutant was suppressed by a mutation of ispA and the mutation improved cell growth of the wild-type at low temperatures
      Title: A mutation of ispA that is involved in isoprenoid biogenesis can improve growth of Escherichia coli at low temperatures.
    3. N-terminus verified by Edman degradation on complete protein
      Title: '98 Escherichia coli SWISS-2DPAGE database update.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables dimethylallyltranstransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables geranyltranstransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables geranyltranstransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables prenyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables prenyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within farnesyl diphosphate biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within geranyl diphosphate biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in isoprenoid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    geranyl diphosphate/farnesyl diphosphate synthase
    NP_414955.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      440202..441101 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414955.1 geranyl diphosphate/farnesyl diphosphate synthase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414955.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7IJV8
      Conserved Domains (1) summary
      PRK10581
      Location:1 → 299
      PRK10581; (2E,6E)-farnesyl diphosphate synthase
    Nucleotide Protein
    Heading Accession and Version

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