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    wrbA NAD(P)H:quinone oxidoreductase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947263, updated on 3-Dec-2024

    Summary

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    Official Symbol
    wrbA
    Official Full Name
    NAD(P)H:quinone oxidoreductase
    Primary source
    ECOCYC:EG11540
    Locus tag
    b1004
    See related
    ASAP:ABE-0003392
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0995
    Summary
    WrbA was originally identified as a stationary phase TrpR-binding protein that affected the interaction of TrpR with its operator sites in vitro (Yang, 1993). [More information is available at EcoGene: EG11540]. The purified WrbA protein has NAD(P)H:quinone oxidoreductase activity . [More information is available at EcoCyc: EG11540].
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    Genomic context

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    Sequence:
    NC_000913.3 (1067112..1067708, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene glucose-1-phosphatase Neighboring gene PF13993 family protein YccJ Neighboring gene stress-induced bacterial acidophilic repeat motifs-containing protein YmdF Neighboring gene pyrimidine:H(+) symporter

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structural organization of WrbA in apo- and holoprotein crystals. Wolfova J, et al. Biochim Biophys Acta, 2009 Sep. PMID 19665595
    2. Spectroelectrochemical investigation of a flavoprotein with a flavin-modified gold electrode. Nöll G, et al. Langmuir, 2006 Feb 28. PMID 16489832
    3. Role of flavin mononucleotide in the thermostability and oligomerization of Escherichia coli stress-defense protein WrbA. Natalello A, et al. Biochemistry, 2007 Jan 16. PMID 17209564
    4. Crystallization and preliminary diffraction analysis of Escherichia coli WrbA in complex with its cofactor flavin mononucleotide. Wolfová J, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2007 Jul 1. PMID 17620713, Free PMC Article
    5. WrbA bridges bacterial flavodoxins and eukaryotic NAD(P)H:quinone oxidoreductases. Carey J, et al. Protein Sci, 2007 Oct. PMID 17893367, Free PMC Article

    See all (28) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Topologically non-equivalent residues undergo remarkably similar local structural changes upon Flavin Mononucleotide binding to WrbA or to flavodoxin, despite differences in multimeric organization and residue types at the binding sites.
      Title: Structural organization of WrbA in apo- and holoprotein crystals.
    2. Structural comparison of WrbA, flavodoxin, and Nqo1 indicates how the twisted open-sheet fold of flavodoxins is elaborated to form multimers that extend catalytic function from one-electron transfer between protein partners.
      Title: WrbA bridges bacterial flavodoxins and eukaryotic NAD(P)H:quinone oxidoreductases.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: A stationary-phase protein of Escherichia coli that affects the mode of association between the trp repressor protein and operator-bearing DNA.
    4. FMN binding has a dramatic effect on WrbA thermal stability, shifting the Tm by approximately 40 degrees C.
      Title: Role of flavin mononucleotide in the thermostability and oligomerization of Escherichia coli stress-defense protein WrbA.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables FMN binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables FMN binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables NAD binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables NAD(P)H dehydrogenase (quinone) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables NAD(P)H dehydrogenase (quinone) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables NAD(P)H dehydrogenase (quinone) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables NAD(P)H dehydrogenase (quinone) activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables NADH:ubiquinone reductase (non-electrogenic) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables NADP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables NADPH dehydrogenase (quinone) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables flavin adenine dinucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within response to oxidative stress IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane HDA PubMed 
    is_active_in membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    NAD(P)H:quinone oxidoreductase

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1067112..1067708 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415524.1 NAD(P)H:quinone oxidoreductase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415524.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6M9WX62, A0A8S7E8G3
      Conserved Domains (1) summary
      PRK03767
      Location:1 → 198
      PRK03767; NAD(P)H:quinone oxidoreductase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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