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    hybO hydrogenase 2 small subunit [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945902, updated on 3-Dec-2024

    Summary

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    Official Symbol
    hybO
    Official Full Name
    hydrogenase 2 small subunit
    Primary source
    ECOCYC:G7554
    Locus tag
    b2997
    See related
    ASAP:ABE-0009834
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2991; yghV
    Summary
    Verified Tat substrate: [More information is available at EcoGene: EG13006]. HybO is the small subunit of hydrogenase 2, and it contains three Fe-S centers . [More information is available at EcoCyc: G7554].
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    Genomic context

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    Sequence:
    NC_000913.3 (3145143..3146261, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene hydrogenase 2 maturation protease Neighboring gene hydrogenase 2 large subunit Neighboring gene hydrogenase 2 membrane subunit Neighboring gene hydrogenase 2 iron-sulfur protein Neighboring gene DUF2623 domain-containing protein YghW Neighboring gene pseudo Neighboring gene protein YqhI

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The CO and CN(-) ligands to the active site Fe in [NiFe]-hydrogenase of Escherichia coli have different metabolic origins. Forzi L, et al. FEBS Lett, 2007 Jul 10. PMID 17597615
    2. Isolation and characterisation of a soluble active fragment of hydrogenase isoenzyme 2 from the membranes of anaerobically grown Escherichia coli. Ballantine SP, et al. Eur J Biochem, 1986 Apr 15. PMID 3516690
    3. Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli. Richard DJ, et al. Microbiology (Reading), 1999 Oct. PMID 10537212
    4. How Escherichia coli is equipped to oxidize hydrogen under different redox conditions. Lukey MJ, et al. J Biol Chem, 2010 Feb 5. PMID 19917611, Free PMC Article
    5. Molecular biology of microbial hydrogenases. Vignais PM, et al. Curr Issues Mol Biol, 2004 Jul. PMID 15119826

    See all (35) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Studies indicate that Hyd-1 and Hyd-2 are two H(2) uptake enzymes, while Hyd-1 is encoded by the first two genes of the hyaABCDEF operon, hyaA and hyaB, Hyd-2 is encoded by genes hybO and hybC of the hybOABCDEFG operon.
      Title: How Escherichia coli is equipped to oxidize hydrogen under different redox conditions.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: Reassignment of the gene encoding the Escherichia coli hydrogenase 2 small subunit--identification of a soluble precursor of the small subunit in a hypB mutant.
    3. The CO and CN(-) ligands to the active site Fe in [NiFe]-hydrogenase of Escherichia coli have different metabolic origins.
      Title: The CO and CN(-) ligands to the active site Fe in [NiFe]-hydrogenase of Escherichia coli have different metabolic origins.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables electron transfer activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ferredoxin hydrogenase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrogenase (acceptor) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables iron-sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables iron-sulfur cluster binding ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within anaerobic respiration EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in anaerobic respiration IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    part_of [Ni-Fe] hydrogenase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of ferredoxin hydrogenase complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane HDA PubMed 
    is_active_in membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in periplasmic space IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    hydrogenase 2 small subunit
    NP_417471.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3145143..3146261 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417471.1 hydrogenase 2 small subunit [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417471.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2X1MSQ3
      Conserved Domains (1) summary
      PRK10468
      Location:1 → 371
      PRK10468; hydrogenase 2 small subunit; Provisional
    Nucleotide Protein
    Heading Accession and Version

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