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    dut dUTP diphosphatase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948607, updated on 3-Dec-2024

    Summary

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    Official Symbol
    dut
    Official Full Name
    dUTP diphosphatase
    Primary source
    ECOCYC:EG10251
    Locus tag
    b3640
    See related
    ASAP:ABE-0011899
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    dnaS; ECK3630; sof
    Summary
    dut is an essential gene. [More information is available at EcoGene: EG10251]. Deoxyuridine triphosphatase (dUTPase) catalyzes the hydrolysis of dUTP, maintaining a low intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. dUTPase is specific for dUTP as a substrate, the active site discriminating between nucleotides with respect to the sugar moiety as well as the pyrimidine base. [More information is available at EcoCyc: EG10251].
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    Genomic context

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    Sequence:
    NC_000913.3 (3813929..3814387)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 50S ribosomal subunit protein L28 Neighboring gene RadC-like JAB domain-containing protein YicR Neighboring gene fused 4'-phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase Neighboring gene nucleoid occlusion factor SlmA Neighboring gene orotate phosphoribosyltransferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Atomic resolution structure of Escherichia coli dUTPase determined ab initio. González A, et al. Acta Crystallogr D Biol Crystallogr, 2001 Jun. PMID 11375495
    2. Transient accumulation of Okazaki fragments as a result of uracil incorporation into nascent DNA. Tye BK, et al. Proc Natl Acad Sci U S A, 1977 Jan. PMID 319455, Free PMC Article
    3. Overproduction and large-scale preparation of deoxyuridine triphosphate nucleotidohydrolase from Escherichia coli. Hoffmann I, et al. Eur J Biochem, 1987 Apr 1. PMID 3030754
    4. Isolation and characterization of the dut gene of Escherichia coli. II. Restriction enzyme mapping and analysis of polypeptide products. Lundberg LG, et al. Gene, 1983 Apr. PMID 6134654
    5. Crystal structure of the Escherichia coli dUTPase in complex with a substrate analogue (dUDP). Larsson G, et al. Nat Struct Biol, 1996 Jun. PMID 8646539

    See all (38) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data conclude that beta-OH of the strictly conserved serine 72 exercises both ground state (GS) destabilization and transition state (TS) stabilization, effects not usually linked to a single residue.
      Title: A double role for a strictly conserved serine: further insights into the dUTPase catalytic mechanism.
    2. Crystal structure of E. coli dUTPase:alpha,beta-methylene-dUDP and E. coli dUTPase:dUDP:Mn complexes were determined
      Title: Methylene substitution at the alpha-beta bridging position within the phosphate chain of dUDP profoundly perturbs ligand accommodation into the dUTPase active site.
    3. influence of the ung, dug, and dut genes in preventing uracil residues from accumulating in E. coli DNA was quantitatively determined
      Title: Quantitative determination of uracil residues in Escherichia coli DNA: Contribution of ung, dug, and dut genes to uracil avoidance.
    4. dUTPase from Drosophila has a similar affinity of binding towards alpha,beta-imino-dUTP as compared to the prokaryotic E. coli enzyme
      Title: Mechanistic studies of dUTPases.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables dUTP diphosphatase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables dUTP diphosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables dUTP diphosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables dUTP diphosphatase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium ion binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in dUMP biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within dUMP biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in dUMP biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in dUMP biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in dUTP catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within dUTP catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in dUTP catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in dUTP catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in nucleotide metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within protein homotrimerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol HDA PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    dUTP diphosphatase
    NP_418097.2

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3813929..3814387
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418097.2 dUTP diphosphatase [Escherichia coli str. K-12 substr. MG1655]

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      B1X974, C4ZXN2
      UniProtKB/TrEMBL
      A0A024LAQ7, A0A236LP18
      Conserved Domains (1) summary
      PRK00601
      Location:2 → 152
      dut; dUTP diphosphatase
    Nucleotide Protein
    Heading Accession and Version

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