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    holD DNA polymerase III subunit psi [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948890, updated on 3-Dec-2024

    Summary

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    Official Symbol
    holD
    Official Full Name
    DNA polymerase III subunit psi
    Primary source
    ECOCYC:EG11414
    Locus tag
    b4372
    See related
    ASAP:ABE-0014340
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4363
    Summary
    The preinitiation complex binds primed ssDNA and loads the beta processivity clamp. [More information is available at EcoCyc: EG11414].
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    Genomic context

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    Sequence:
    NC_000913.3 (4607803..4608216)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene tRNA-Leu Neighboring gene 16S rRNA m(2)G1207 methyltransferase Neighboring gene protein N-acetyltransferase RimI Neighboring gene pyrimidine 5'-nucleotidase YjjG Neighboring gene peptide chain release factor RF3

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Identification, isolation, and overexpression of the gene encoding the psi subunit of DNA polymerase III holoenzyme. Carter JR, et al. J Bacteriol, 1993 Sep. PMID 8366044, Free PMC Article
    2. Mutations Affecting Potassium Import Restore the Viability of the Escherichia coli DNA Polymerase III holD Mutant. Durand A, et al. PLoS Genet, 2016 Jun. PMID 27280472, Free PMC Article
    3. ssb gene duplication restores the viability of ΔholC and ΔholD Escherichia coli mutants. Duigou S, et al. PLoS Genet, 2014 Oct. PMID 25329071, Free PMC Article
    4. DNA polymerase III accessory proteins. III. holC and holD encoding chi and psi. Xiao H, et al. J Biol Chem, 1993 Jun 5. PMID 8389364
    5. Structure of the SSB-DNA polymerase III interface and its role in DNA replication. Marceau AH, et al. EMBO J, 2011 Aug 19. PMID 21857649, Free PMC Article

    See all (87) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. although lagging-strand synthesis is still perturbed in holD trkA mutants, the trkA mutation allows HolD-less Pol III HE to resist increased levels of the SOS-induced bypass polymerase DinB
      Title: Mutations Affecting Potassium Import Restore the Viability of the Escherichia coli DNA Polymerase III holD Mutant.
    2. Results support the idea that controlling the balance between SSB binding modes is critical for DNA Pol III holoenzyme stability.
      Title: ssb gene duplication restores the viability of ΔholC and ΔholD Escherichia coli mutants.
    3. By stabilizing a conformation of the clamp loader that is consistent with the ATPase spiral observed upon DNA binding, psi binding promotes the clamp-loading activity of the complex.
      Title: The mechanism of ATP-dependent primer-template recognition by a clamp loader complex.
    4. N-terminus verified by Edman degradation on mature peptide
      Title: DNA polymerase III accessory proteins. III. holC and holD encoding chi and psi.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3'-5' exonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-directed DNA polymerase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-directed DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in DNA-templated DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within response to radiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DNA polymerase III complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of DNA polymerase III, clamp loader complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of replisome NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA polymerase III subunit psi

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4607803..4608216
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418789.1 DNA polymerase III subunit psi [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418789.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2H4TQW6
      Conserved Domains (1) summary
      PRK06856
      Location:2 → 129
      PRK06856; DNA polymerase III subunit psi; Validated
    Nucleotide Protein
    Heading Accession and Version

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