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    ccmE periplasmic heme chaperone [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946697, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ccmE
    Official Full Name
    periplasmic heme chaperone
    Primary source
    ECOCYC:EG12055
    Locus tag
    b2197
    See related
    ASAP:ABE-0007271
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2189; yejS
    Summary
    CcmE makes a transient covalent linkage to heme at His-130 and then transfers heme to apocytochrome C in the presence of CcmFGH. [More information is available at EcoGene: EG12055]. CcmE is the periplasmic heme chaperone responsible for shuttling heme from the CcmABCDE protoheme IX ABC transport complex to the CcmEFGH holocytochrome c synthetase for attachment to c-type cytochromes. [More information is available at EcoCyc: EG12055].
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    Genomic context

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    Sequence:
    NC_000913.3 (2294901..2295380, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional dual regulator NarP Neighboring gene holocytochrome c synthase CcmH component Neighboring gene thiol:disulfide oxidoreductase CcmG Neighboring gene holocytochrome c synthase CcmF component Neighboring gene cytochrome c maturation protein D Neighboring gene cytochrome c maturation protein C Neighboring gene cytochrome c maturation protein B Neighboring gene cytochrome c maturation protein A

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The CcmE protein of the c-type cytochrome biogenesis system: unusual in vitro heme incorporation into apo-CcmE and transfer from holo-CcmE to apocytochrome. Daltrop O, et al. Proc Natl Acad Sci U S A, 2002 Jul 23. PMID 12119398, Free PMC Article
    2. Interaction of heme with variants of the heme chaperone CcmE carrying active site mutations and a cleavable N-terminal His tag. Stevens JM, et al. J Biol Chem, 2003 Jun 6. PMID 12657624
    3. The C-terminal flexible domain of the heme chaperone CcmE is important but not essential for its function. Enggist E, et al. J Bacteriol, 2003 Jul. PMID 12813076, Free PMC Article
    4. The CcmC:heme:CcmE complex in heme trafficking and cytochrome c biosynthesis. Richard-Fogal C, et al. J Mol Biol, 2010 Aug 20. PMID 20599545, Free PMC Article
    5. A variant System I for cytochrome c biogenesis in archaea and some bacteria has a novel CcmE and no CcmH. Allen JW, et al. FEBS Lett, 2006 Sep 4. PMID 16920107

    See all (66) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. CcmE only interacts stably with CcmC when heme is present; thus, specific residues in each protein provide sites of interaction with heme to form this very stable complex
      Title: The CcmC:heme:CcmE complex in heme trafficking and cytochrome c biosynthesis.
    2. N-terminus verified by Edman degradation on complete protein
      Title: The CcmE protein from Escherichia coli is a haem-binding protein.
    3. role of covalent heme binding to CcmE during the maturation of c-type cytochromes
      Title: The interaction of covalently bound heme with the cytochrome c maturation protein CcmE.
    4. Data show that a variant System I for cytochrome c biogenesis in archaea and some bacteria has a novel CcmE and no CcmH.
      Title: A variant System I for cytochrome c biogenesis in archaea and some bacteria has a novel CcmE and no CcmH.
    5. In the absence of ATP hydrolysis by mutant CcmA, heme chaperone CcmE is incompetent for heme transfer to an apocytochrome c or for release into the periplasm as a soluble variant.
      Title: Loss of ATP hydrolysis activity by CcmAB results in loss of c-type cytochrome synthesis and incomplete processing of CcmE.
    6. Holo(haem)CcmE binds to CcmC in a stable dead-end complex, indicating high affinity binding of haem to CcmC.
      Title: ABC transporter-mediated release of a haem chaperone allows cytochrome c biogenesis.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables heme binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables heme binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within cytochrome c biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within cytochrome c biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cytochrome complex assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cytochrome complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in heme import across plasma membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of ATP-binding cassette (ABC) transporter complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    periplasmic heme chaperone

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2294901..2295380 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416701.1 periplasmic heme chaperone [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416701.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7A2LVF3
      Conserved Domains (1) summary
      PRK13150
      Location:1 → 159
      PRK13150; cytochrome c maturation protein CcmE
    Nucleotide Protein
    Heading Accession and Version

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